Metaphase kinetochore movements are regulated by kinesin-8 motors and microtubule dynamic instability

Molecular Biology of the Cell
Anna H KlemmIva M Tolić

Abstract

During metaphase, sister chromatids are connected to microtubules extending from the opposite spindle poles via kinetochores to protein complexes on the chromosome. Kinetochores congress to the equatorial plane of the spindle and oscillate around it, with kinesin-8 motors restricting these movements. Yet, the physical mechanism underlying kinetochore movements is unclear. We show that kinetochore movements in the fission yeast Schizosaccharomyces pombe are regulated by kinesin-8-promoted microtubule catastrophe, force-induced rescue, and microtubule dynamic instability. A candidate screen showed that among the selected motors only kinesin-8 motors Klp5/Klp6 are required for kinetochore centering. Kinesin-8 accumulates at the end of microtubules, where it promotes catastrophe. Laser ablation of the spindle resulted in kinetochore movement toward the intact spindle pole in wild-type and klp5Δ cells, suggesting that kinetochore movement is driven by pulling forces. Our theoretical model with Langevin description of microtubule dynamic instability shows that kinesin-8 motors are required for kinetochore centering, whereas sensitivity of rescue to force is necessary for the generation of oscillations. We found that irregular kinetoc...Continue Reading

References

Jul 1, 1992·The Journal of Cell Biology·K L McDonaldJ R McIntosh
Jan 1, 1991·Cell Motility and the Cytoskeleton·D WiseE D Salmon
Jan 1, 1988·Annual Review of Biophysics and Biophysical Chemistry·R B Nicklas
Feb 16, 1995·Nature·X Li, R B Nicklas
Nov 2, 2001·The EMBO Journal·S M Kim, J A Huberman
Jun 25, 2002·Biophysical Journal·Ajit P Joglekar, Alan J Hunt
May 14, 2003·Fungal Genetics and Biology : FG & B·Conrad L SchochB Gillian Turgeon
May 29, 2003·Biophysical Journal·Brian L SpragueDavid J Odde
Nov 20, 2003·Journal of Cell Science·Meredith Johnson SagollaW Zacheus Cande
Jul 10, 2004·Current Biology : CB·Iva M Tolić-NørrelykkeFrancesco S Pavone
Aug 7, 2004·Current Biology : CB·Alexey KhodjakovFred Chang
Dec 2, 2004·Nature Cell Biology·Sabina ZimmermanFred Chang
Feb 4, 2005·Molecular Biology of the Cell·Isabelle LoïodiceP T Tran
Nov 18, 2005·Nature·Ekaterina L GrishchukJ Richard McIntosh
Feb 25, 2006·Yeast·Susan L Forsburg, Nicholas Rhind
Aug 15, 2006·Nature Cell Biology·Vladimir VargaJonathon Howard
Feb 20, 2007·Current Biology : CB·Sven K VogelIva Tolić-Nørrelykke
Jun 15, 2007·Biology of the Cell·Thibault CourtheouxSylvie Tournier
Aug 31, 2007·Nature·Tobias ReichenbachErwin Frey
Dec 22, 2007·Nature Reviews. Molecular Cell Biology·Iain M Cheeseman, Arshad Desai
Jun 3, 2008·Trends in Cell Biology·Melissa K GardnerKerry Bloom
Sep 11, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jian LiuTerence Hwa
Mar 19, 2009·Molecular Systems Biology·Christian TischerMarileen Dogterom
Apr 2, 2009·Journal of Microscopy·I RaabeI M Tolić-Nørrelykke
Apr 18, 2009·Cell Motility and the Cytoskeleton·Lynne CassimerisBruce Carney
Apr 23, 2009·Biophysical Journal·L E HoughM D Betterton
Dec 2, 2009·The Journal of Cell Biology·Thibault CourtheouxSylvie Tournier
Jan 12, 2010·Current Opinion in Cell Biology·Iva M Tolić-Nørrelykke

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Citations

Nov 26, 2020·Developmental Cell·Nenad Pavin, Iva M Tolić
Jan 29, 2021·Molecular Biology of the Cell·Iva M Tolić, Nenad Pavin
Apr 4, 2021·International Journal of Molecular Sciences·Ayumu Yamamoto
May 26, 2021·Current Biology : CB·Patrik RisteskiIva M Tolić
Jul 27, 2021·Cytoskeleton·Alexandra CiorîțăAnita Jannasch

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Methods Mentioned

BETA
PCR
confocal microscopy

Software Mentioned

Andor iQ2
MATLAB
Andor iQ
Low Light Tracking
Fiji

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