Abstract
Metastasis-associated in colon cancer-1 (MACC1) is a newly identified gene that is involved in the development and progression of hepatocellular carcinoma (HCC), however its investigation has not been comprehensive. In the present study, in vitro techniques, including immunohistochemistry, western blotting, reverse transcription quantitative polymerase chain reaction, metabolic assay, MTT assay, colony formation assay and prognostic analysis were used to confirm the involvement of MACC1 in HCC. Histological examination confirmed that the protein expression of MACC1 was upregulated in HCC and was associated with the hexokinase-2 (HK2) protein, which also indicates a poor prognosis. Knockdown of MACC1 induced the reduction of glycogen consumption and lactate production, which then lead to a marked reduction of proliferation in the MHCC-97H cells. However, the overexpression of MACC1 produced the opposite results in the HepG2 cells. These results suggested that MACC1 leads to a poor prognosis in HCC, partly by promoting proliferation via enhancement in glucose metabolism by HK2. Therefore, this pathway has the potential to become an important therapeutic target in HCC.
References
Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·E Bustamante, P L Pedersen
Apr 1, 1989·Scandinavian Journal of Gastroenterology·I AlbersW Creutzfeldt
Aug 1, 1997·Journal of Bioenergetics and Biomembranes·S P MathupalaP L Pedersen
Jun 8, 2001·Genes & Development·K GottlobN Hay
Jul 23, 2003·Current Medicinal Chemistry·John G Pastorino, Jan B Hoek
Jan 10, 2004·Journal of Neurochemistry·Gautam N Bijur, Richard S Jope
Feb 16, 2005·Journal of Hepatology·Geum-Youn GwakGregory J Gores
Mar 11, 2005·Best Practice & Research. Clinical Gastroenterology·Hubert E Blum
Mar 12, 2005·CA: a Cancer Journal for Clinicians·D Max ParkinPaola Pisani
Jul 28, 2006·Cold Spring Harbor Symposia on Quantitative Biology·D C Wallace
Feb 10, 2007·Current Oncology Reports·Martin Sattler, Ravi Salgia
Dec 27, 2007·Acta Pharmacologica Sinica·Feng LiuXi-rong Guo
Dec 23, 2008·Nature Medicine·Ulrike SteinPeter M Schlag
Aug 12, 2009·The International Journal of Biochemistry & Cell Biology·Franziska Arlt, Ulrike Stein
Aug 20, 2009·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Keun Jae AhnJong Doo Lee
Nov 26, 2010·The Journal of Thoracic and Cardiovascular Surgery·Hidehiko ShimokawaKosei Yasumoto
Oct 1, 2011·Journal of Translational Medicine·Jiliang QiuYunfei Yuan
Jan 19, 2012·BMC Cancer·Alois H LangAxel Muendlein
Dec 4, 2012·Molecular Endocrinology·Yi ZhuE Dale Abel
Dec 13, 2012·Molecular and Cellular Biochemistry·Jie GaoYingmin Yao
Jun 1, 2011·Chinese Journal of Cancer Research = Chung-kuo Yen Cheng Yen Chiu·Shao-Hua GeJia-Fu Ji
Mar 19, 2013·Cell & Bioscience·Yongbo HuangMengfeng Li
May 1, 2013·Journal of Cellular Physiology·Catherine L Neary, John G Pastorino
May 30, 2013·PloS One·Chan XieLibing Song
Jul 10, 2013·The Journal of Biological Chemistry·David J RobertsShigeki Miyamoto
Citations
Aug 8, 2018·Cell Death & Disease·Hwa Jin KimJeong Hoon Kim
Sep 26, 2017·Oncology Letters·He LiHong-Hao Zhou
Apr 19, 2017·Oncotarget·Jiayuan WuTaiping He
Oct 30, 2016·Oncotarget·Weige TanWenbo Zheng
Oct 30, 2018·Journal of Cellular and Molecular Medicine·Aifen LinWei-Hua Yan
Jul 8, 2020·Journal of Experimental & Clinical Cancer Research : CR·Jiao FengChuanyong Guo
Jan 5, 2019·Cancer Metastasis Reviews·Harikrishnan RadhakrishnanUlrike Stein
May 12, 2018·Current Cancer Drug Targets·A P AlvesC J Maia
Jul 19, 2019·Experimental and Therapeutic Medicine·Xi OuJi-Kui Liu
Feb 26, 2019·Frontiers in Oncology·Aifen LinWei-Hua Yan
Mar 4, 2021·Cancers·Jan LisecUlrike Stein
Jun 12, 2021·OncoTargets and Therapy·Yaqian NiuChe Chen