Metastasis-associated microRNA expression in canine uveal melanoma

Veterinary and Comparative Oncology
M P StarkeyR Dubielzig

Abstract

Uveal melanoma (UM) is the most common primary intraocular tumour in dogs. There is no effective means of predicting whether a tumour will metastasize. microRNA (miRNA) metastasis signatures have been identified for several human cancers, including UM. In this study we investigated whether metastasizing and non-metastasizing canine UMs can be distinguished by miRNA expression levels. miRNA microarray profiling was used to compare miRNA expression in 8 metastasizing and 12 non-metastasizing formalin-fixed, paraffin-embedded (FFPE) primary UM biopsies. Fourteen miRNAs exhibited statistically significant differences in expression between the metastasizing and non-metastasizing tumours. Class prediction analysis pinpointed 9 miRNAs which categorized tumours as metastasizing or non-metastasizing with an accuracy of 89%. Of the discriminating miRNAs, 8 were up-regulated in metastasizing UM, and included 3 miRNAs implicated as potential "metastasis activators" in human cutaneous melanoma. The expression of 4 of the miRNAs was subsequently measured using the quantitative reverse transcription polymerase chain reaction (RT-qPCR), and their up-regulation in metastasizing tumours validated. miRNA expression profiles may potentially be use...Continue Reading

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Citations

Nov 7, 2019·Clinical & Experimental Metastasis·Louise van der WeydenGeoffrey A Wood
Jan 19, 2019·Oncology Letters·Norio UshioNaoki Miura
Apr 10, 2018·Cancer Biomarkers : Section a of Disease Markers·Yuanyu WuXuedong Fang
Jul 17, 2020·OncoTargets and Therapy·Yi Fan LiWen Bin Wei
Oct 3, 2020·Frontiers in Veterinary Science·Ashkan FaridiMajid Fasihi Harandi
Feb 11, 2019·Clinical & Experimental Metastasis·Bushra Abu-Helil, Louise van der Weyden
Nov 17, 2020·Frontiers in Oncology·Pedro Luiz Porfirio XavierHeidge Fukumasu

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