Metastatic potential of mouse Lewis lung cancer cells is regulated via ganglioside GM1 by modulating the matrix metalloprotease-9 localization in lipid rafts

The Journal of Biological Chemistry
Qing ZhangKoichi Furukawa

Abstract

To analyze mechanisms for cancer metastasis, we established high metastatic sublines from mouse Lewis lung cancer (P29) by repeated injection. Sublines established from the two subclones H7 and C4 commonly exhibited increased proliferation and invasion activity and reduced expression of ganglioside GM1, although they showed different preferences in their target organs of metastasis. The high metastatic sublines secreted higher levels of activated matrix metalloprotease (MMP)-9 as well as pro-MMP-9 in the culture medium than the parent lines. Furthermore, they contained MMP-9 at the glycolipid-enriched microdomain (GEM)/rafts fractionated by the sucrose density gradient ultracentrifugation of Triton X-100 extracts, whereas the parent cells showed faint bands at the fraction. When high metastatic sublines were treated with methyl-beta-cyclodextrin, their invasion activities were dramatically suppressed, and the MMP-9 secretion was also suppressed. All these results indicated that GEM/rafts play crucial roles in the increased invasion and high metastatic potential. To clarify the implication of reduced GM1 expression, low GM1-expressing cell lines were established using an RNA interference-expression vector of the GM1 synthase. Lo...Continue Reading

References

Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·H E Van Wart, H Birkedal-Hansen
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·A J KoleskeM P Lisanti
Oct 1, 1993·Journal of Cellular Physiology·H LarjavaJ Heino
Oct 1, 1998·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·J WizeS Maśliński
Jan 19, 1999·Annual Review of Cell and Developmental Biology·D A Brown, E London
Mar 17, 1999·International Journal of Cancer. Journal International Du Cancer·M AgrezE W Howard
May 13, 1999·The Journal of Biological Chemistry·J ZhaoK Furukawa
Dec 20, 2000·Experimental Cell Research·A PuyraimondS Menashi
May 8, 2001·International Journal of Cancer. Journal International Du Cancer·G J ThomasP M Speight
Jun 20, 2001·Nature Reviews. Molecular Cell Biology·K Simons, D Toomre
Jul 18, 2001·The Journal of Biological Chemistry·B RazaniM P Lisanti
May 7, 2002·Nature Reviews. Cancer·Mikala Egeblad, Zena Werb
Aug 1, 2002·Proceedings of the National Academy of Sciences of the United States of America·Senitiroh Hakomori
Aug 15, 2002·Laboratory Investigation; a Journal of Technical Methods and Pathology·Panagiotis M KaramessinisEffie C Tsilibary
Oct 2, 2002·The Journal of Biological Chemistry·Xiao-Qi WangAmy S Paller
Nov 28, 2002·International Journal of Cancer. Journal International Du Cancer·Ho-Hsiang ChenKoichi Furukawa
May 2, 2003·The Journal of Biological Chemistry·Xiao-Qi WangAmy S Paller
Jun 6, 2003·Cancer Metastasis Reviews·Rafael FridmanShahriar Mobashery
Jul 23, 2003·Cancer·S David Nathanson
Jul 23, 2003·Proceedings of the National Academy of Sciences of the United States of America·Melanie RolliBrunhilde Felding-Habermann
Feb 10, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Neal S FedarkoLarry W Fisher
May 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michael StefanidakisErkki Koivunen
Jun 19, 2004·Molecular Membrane Biology·Alex J Laude, Ian A Prior
Dec 30, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Larry W FisherNeal S Fedarko
Feb 5, 2005·American Journal of Physiology. Cell Physiology·Terence M Williams, Michael P Lisanti

❮ Previous
Next ❯

Citations

Jul 6, 2012·Glycoconjugate Journal·Koichi FurukawaKeiko Furukawa
May 25, 2012·Journal of Biochemistry·Koichi FurukawaKeiko Furukawa
Aug 17, 2012·Future Oncology·Catherine RabuLindy Durrant
Aug 26, 2014·American Journal of Respiratory and Critical Care Medicine·Yi-Fang YangMichael Hsiao
May 13, 2011·Neurochemical Research·Koichi FurukawaKeiko Furukawa
Mar 13, 2016·Biochimica Et Biophysica Acta·Joanne ChiaFrederic Bard
Jan 30, 2014·International Journal of Cancer. Journal International Du Cancer·Anne-Marie BleauLuis M Montuenga
Jul 18, 2009·Molecular and Cellular Neurosciences·Zuzana SiskováWia Baron
Nov 14, 2014·Nucleic Acids Research·Anup ShahMichelle M Hill
Dec 9, 2014·Archives of Medical Research·Miklós VecsernyésÉva Fenyvesi
Nov 22, 2018·PloS One·Yuhsuke OhmiKeiko Furukawa
Aug 9, 2016·International Journal of Oncology·Kenichi NogimoriKoichi Furukawa
Sep 26, 2006·The Biochemical Journal·Severine Van Slambrouck, Wim F A Steelant
Jul 22, 2021·Molecular Oncology·Antonia PiazzesiGerhild van Echten-Deckert
Feb 7, 2012·Biochemical and Biophysical Research Communications·Yasuyuki MatsumotoKoichi Furukawa

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.