Metazoan Ribosome Inactivating Protein encoding genes acquired by Horizontal Gene Transfer

Scientific Reports
Walter J LapadulaMaximiliano Juri Ayub

Abstract

Ribosome inactivating proteins (RIPs) are RNA N-glycosidases that depurinate a specific adenine residue in the conserved sarcin/ricin loop of 28S rRNA. These enzymes are widely distributed among plants and their presence has also been confirmed in several bacterial species. Recently, we reported for the first time in silico evidence of RIP encoding genes in metazoans, in two closely related species of insects: Aedes aegypti and Culex quinquefasciatus. Here, we have experimentally confirmed the presence of these genes in mosquitoes and attempted to unveil their evolutionary history. A detailed study was conducted, including evaluation of taxonomic distribution, phylogenetic inferences and microsynteny analyses, indicating that mosquito RIP genes derived from a single Horizontal Gene Transfer (HGT) event, probably from a cyanobacterial donor species. Moreover, evolutionary analyses show that, after the HGT event, these genes evolved under purifying selection, strongly suggesting they play functional roles in these organisms.

References

Jul 1, 1987·The American Journal of Tropical Medicine and Hygiene·F H CollinsV Finnerty
Dec 5, 1998·Microbiology and Molecular Biology Reviews : MMBR·S RazinY Naot
Jan 28, 1999·The Journal of Biological Chemistry·K A HudakN E Tumer
Mar 10, 2001·Nature·E S LanderUNKNOWN International Human Genome Sequencing Consortium
Oct 12, 2001·Toxicon : Official Journal of the International Society on Toxinology·K Sandvig
Nov 26, 2002·Molecular Biology and Evolution·J Peter GogartenJeffrey G Lawrence
Aug 7, 2003·Proceedings of the National Academy of Sciences of the United States of America·C G KurlandOtto G Berg
Jun 3, 2004·Genome Research·Gavin E CrooksSteven E Brenner
Feb 11, 2005·Molecular Biology and Evolution·Sergei L Kosakovsky Pond, Simon D W Frost
Nov 8, 2008·Molecular Biology and Evolution·Megan WoolfitScott L O'Neill
Jan 22, 2009·BMC Genomics·Lisa KlassonSteven P Sinkins
Jul 31, 2010·Bioinformatics·Wayne DelportSergei L Kosakovsky Pond
Sep 30, 2010·Proceedings of the National Academy of Sciences of the United States of America·Etienne G J DanchinPierre Abad
Feb 8, 2011·PLoS Genetics·Todd J Treangen, Eduardo P C Rocha
Feb 22, 2011·Trends in Genetics : TIG·Julie C Dunning Hotopp
Feb 22, 2011·Bioinformatics·Diego DarribaDavid Posada
May 5, 2011·Molecular Plant-microbe Interactions : MPMI·Annelies HaegemanEtienne G J Danchin
May 20, 2011·Nucleic Acids Research·Robert D FinnSean R Eddy
Jan 17, 2012·Toxicon : Official Journal of the International Society on Toxinology·Walter J LapadulaMaximiliano Juri Ayub
Feb 24, 2012·Systematic Biology·Fredrik RonquistJohn P Huelsenbeck
Apr 10, 2012·Drug Discovery Today·Munish PuriRaghbir C Gupta
May 22, 2013·Parasites & Vectors·Guillaume MinardClaire Valiente Moro
Sep 17, 2013·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Jinling Huang
Oct 10, 2013·Molecular Biology and Evolution·Bo Xu, Ziheng Yang
Aug 20, 2015·Nature·Chuan KuWilliam F Martin
Nov 26, 2015·Proceedings of the National Academy of Sciences of the United States of America·Thomas C BoothbyBob Goldstein
Dec 30, 2015·Proceedings of the National Academy of Sciences of the United States of America·Phineas T HamiltonSteve J Perlman
Apr 2, 2016·Proceedings of the National Academy of Sciences of the United States of America·Georgios KoutsovoulosMark Blaxter
Apr 17, 2016·Proceedings of the National Academy of Sciences of the United States of America·Thomas A Richards, Adam Monier
Jun 17, 2016·Genome Biology and Evolution·Nicky WybouwThomas Van Leeuwen

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Citations

Jun 28, 2017·Toxicon : Official Journal of the International Society on Toxinology·Walter Jesús Lapadula, Maximiliano Juri Ayub
Sep 25, 2020·Scientific Reports·Walter J LapadulaMaximiliano Juri Ayub
Mar 21, 2020·Applied Microbiology and Biotechnology·Jack Ho WongLixin Xia

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Datasets Mentioned

BETA
KXJ73133
KXJ73764
EU211398
KX674698

Methods Mentioned

BETA
RIP
PCR
RIPs

Software Mentioned

CodeML
ProtTest3
PAL2NAL
PhyML
MAFFT
BLASTp
BLASTx
Datamonkey
Fasta
Weblogo

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