Metformin Affects Paclitaxel Sensitivity of Ovarian Cancer Cells Through Autophagy Mediated by Long Noncoding RNASNHG7/miR-3127-5p Axis

Cancer Biotherapy & Radiopharmaceuticals
Ze YuJunwei Zhai

Abstract

Background: Ovarian cancer is the public health issue worldwide. Paclitaxel is a first-line chemotherapy drug for ovarian cancer, but the paclitaxel resistance weakens the therapeutic effect. Metformin (Met) improved the paclitaxel sensitivity in a mouse model of ovarian cancer. However, the mechanism of Met on paclitaxel sensitivity is still unclear in ovarian cancer. Methods: Cell viability, apoptosis, migration, and invasion were measured by Cell Counting Kit-8 (CCK8), flow cytometry, and transwell assays severally. The expression of long noncoding RNA (lncRNA) small nucleolar RNA host gene 7 (SNHG7) and microRNA-3127-5p (miR-3127-5p) were detected by real-time quantitative polymerase chain reaction. The protein levels of poly (ADP-ribose) polymerase, microtubule-associated protein 1 light chain 3 (LC3)-I, LC3-II, and Beclin 1 were examined by western blot assay. RNA immunoprecipitation assay detected the relationship between SNHG7 and miR-3127-5p. Then, the binding correlation between SNHG7 and miR-3127-5p was predicted by starBase and verified by the dual-luciferase reporter. The effects of Met and SNHG7 on tumor growth were tested in ovarian cancer mice model. Results: Met inhibited cell viability, migration, invasion, SN...Continue Reading

References

Jul 27, 2004·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Jean-Christophe AméGilbert de Murcia
Mar 25, 2005·Nature Reviews. Drug Discovery·Dong Wang, Stephen J Lippard
Apr 30, 2013·Asian Pacific Journal of Cancer Prevention : APJCP·Cigir Biray AvciCumhur Gunduz
Apr 17, 2014·Current Medicinal Chemistry·Hongping Xia, Kam M Hui
Jun 27, 2014·Seminars in Cell & Developmental Biology·Je-Hyun YoonMyriam Gorospe
Dec 3, 2014·American Journal of Obstetrics and Gynecology·Ernst LengyelIris L Romero
Aug 26, 2016·Nature Reviews. Disease Primers·Ursula A MatulonisBeth Y Karlan
Oct 21, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Marie DauganVincent Boudy
Jul 14, 2017·Journal of Cellular Physiology·Bornali DebSupriyo Chakraborty
Sep 20, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Weibo XuJunqing Hou
Jan 10, 2018·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Jan 18, 2018·Acta Biochimica Et Biophysica Sinica·Nipun Saini, Xiaohe Yang
Feb 17, 2018·Biochemical and Biophysical Research Communications·Kyung Jin EohEun Ji Nam
May 5, 2018·Journal of Cellular and Molecular Medicine·Dongfang TangYifeng Sun
Jul 5, 2018·Journal of Cellular and Molecular Medicine·Ruoming WangJingjing Seng
Sep 8, 2018·Journal of Cellular Physiology·Peiwen LiZhenning Wang
Apr 26, 2019·Diabetes/metabolism Research and Reviews·Alexey V ZilovImran Paruk

❮ Previous
Next ❯

Citations

Apr 15, 2021·Cancer Cell International·Nahid AlimoradiReihaneh Fatehi

❮ Previous
Next ❯

Datasets Mentioned

BETA
A2780

Methods Mentioned

BETA
electrophoresis
transfection
immunoprecipitation
xenograft
flow cytometry
RIP
xenografts

Software Mentioned

starBase
SPSS
GraphPad Prism

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms