Abstract
Background: Ovarian cancer is the public health issue worldwide. Paclitaxel is a first-line chemotherapy drug for ovarian cancer, but the paclitaxel resistance weakens the therapeutic effect. Metformin (Met) improved the paclitaxel sensitivity in a mouse model of ovarian cancer. However, the mechanism of Met on paclitaxel sensitivity is still unclear in ovarian cancer. Methods: Cell viability, apoptosis, migration, and invasion were measured by Cell Counting Kit-8 (CCK8), flow cytometry, and transwell assays severally. The expression of long noncoding RNA (lncRNA) small nucleolar RNA host gene 7 (SNHG7) and microRNA-3127-5p (miR-3127-5p) were detected by real-time quantitative polymerase chain reaction. The protein levels of poly (ADP-ribose) polymerase, microtubule-associated protein 1 light chain 3 (LC3)-I, LC3-II, and Beclin 1 were examined by western blot assay. RNA immunoprecipitation assay detected the relationship between SNHG7 and miR-3127-5p. Then, the binding correlation between SNHG7 and miR-3127-5p was predicted by starBase and verified by the dual-luciferase reporter. The effects of Met and SNHG7 on tumor growth were tested in ovarian cancer mice model. Results: Met inhibited cell viability, migration, invasion, SN...Continue Reading
References
Jul 9, 1999·Genomics·V M AitaB Levine
Nov 4, 2000·The EMBO Journal·Y KabeyaT Yoshimori
Jul 27, 2004·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Jean-Christophe AméGilbert de Murcia
Mar 25, 2005·Nature Reviews. Drug Discovery·Dong Wang, Stephen J Lippard
Sep 21, 2007·Cancer Treatment Reviews·Britta StordalRoss Davey
Jul 14, 2010·Gynecologic Oncology·Zhimin LiZehua Wang
Aug 13, 2010·Nature·Huili GuoDavid P Bartel
Apr 30, 2013·Asian Pacific Journal of Cancer Prevention : APJCP·Cigir Biray AvciCumhur Gunduz
Apr 17, 2014·Current Medicinal Chemistry·Hongping Xia, Kam M Hui
Jun 27, 2014·Seminars in Cell & Developmental Biology·Je-Hyun YoonMyriam Gorospe
Oct 7, 2014·Scientific Reports·Yifeng SunWen Gao
Dec 3, 2014·American Journal of Obstetrics and Gynecology·Ernst LengyelIris L Romero
Jan 22, 2015·Autophagy·Song-Fa ZhangXing Xie
May 23, 2015·Diabetes·Yuichi HattoriToshio Hayashi
Aug 26, 2016·Nature Reviews. Disease Primers·Ursula A MatulonisBeth Y Karlan
Oct 21, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Marie DauganVincent Boudy
Oct 19, 2016·Autophagy·Mihwa KimDae Joon Kim
Jul 14, 2017·Journal of Cellular Physiology·Bornali DebSupriyo Chakraborty
Jul 21, 2017·Cell Death & Disease·Anna PagottoAlberto Amadori
Jul 29, 2017·Cell·TaChung YuJing-Yuan Fang
Sep 20, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Weibo XuJunqing Hou
Jan 10, 2018·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Jan 13, 2018·Archives of Gynecology and Obstetrics·Di WuJinwei Miao
Jan 13, 2018·Endocrine·Yuan QinChanghao Wu
Jan 18, 2018·Acta Biochimica Et Biophysica Sinica·Nipun Saini, Xiaohe Yang
Feb 17, 2018·Biochemical and Biophysical Research Communications·Kyung Jin EohEun Ji Nam
May 5, 2018·Journal of Cellular and Molecular Medicine·Dongfang TangYifeng Sun
Jul 5, 2018·Journal of Cellular and Molecular Medicine·Ruoming WangJingjing Seng
Sep 8, 2018·Journal of Cellular Physiology·Peiwen LiZhenning Wang
Oct 15, 2018·Journal of Cellular Physiology·Bailin PanLongbiao Xu
Feb 21, 2019·Journal of Drug Targeting·Baiying LiXinhai Zhu
Mar 12, 2019·Frontiers in Pharmacology·Ping WuSuping Zhao
Apr 26, 2019·Diabetes/metabolism Research and Reviews·Alexey V ZilovImran Paruk
Apr 30, 2019·Cancer Cell·Mohamed ElgendySaverio Minucci