Metformin Alleviates the Bone Loss Induced by Ketogenic Diet: An In Vivo Study in Mice

Calcified Tissue International
Qi LiuQingan Zhu

Abstract

Metformin (Met), an anti-diabetes drug, has also shown therapeutic effects for ovariectomy-induced (OVX) osteoporosis. However, similar effects against bone loss induced by a ketogenic diet (KD) have not been tested. This study was aimed to evaluate the microarchitectures and biomechanics of KD-induced osteoporosis with and without administration of Met, and compare the effect of Met on bone loss induced by KD with OVX. Forty female C57BL/6J mice were randomly divided into Sham, OVX, OVX + Met (100 mg/kg/day), KD (3:1 ratio of fat to carbohydrate and protein), and KD + Met (100 mg/kg/day) groups. After 12 weeks, the bone mass and biomechanics were measured in distal cancellous bone and in mid-shaft cortical bone of the femur. The activities of serum alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP), together with immunohistochemistry staining of osteocalcin (OCN) and TRAP, were evaluated. Both OVX and KD induced significant bone loss and compromised biomechanical properties in the cancellous bone, but no effect was found in cortical bone. The administration of Met increased the cancellous bone volume fraction (BV/TV) from 3.78 to 5.23% following the OVX and from 4.04 to 6.33% following the KD, it also en...Continue Reading

References

Aug 24, 1979·Calcified Tissue International·T J HahnD C DeVivo
Dec 1, 1991·Bone and Mineral·D N Kalu
Jul 1, 1993·Bone·C H Turner, D B Burr
May 1, 1997·The Journal of Nutrition·P G Reeves
Mar 28, 2006·European Journal of Pharmacology·Ana M CortizoLeón Schurman
Dec 10, 2008·The American Journal of Clinical Nutrition·A G Christina BergqvistBabette S Zemel
Jun 19, 2009·Journal of Child Neurology·Eric H KossoffJong M Rho
Jul 25, 2009·Journal of Diabetes and Its Complications·Donghu ZhenXulei Tang
Aug 6, 2009·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Maximilian BielohubyMartin Bidlingmaier
Nov 18, 2009·Diabetes Care·Gijs W D LandmanHenk J G Bilo
Mar 24, 2010·European Journal of Pharmacology·Ying GaoJing Hu
Jun 10, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Mary L BouxseinRalph Müller
May 28, 2011·Journal of Cellular Biochemistry·Qi-Guang MaiXiao-Chun Bai
Apr 18, 2012·Frontiers in Pharmacology·Carl E Stafstrom, Jong M Rho
Jun 27, 2013·European Journal of Clinical Nutrition·A PaoliK A Grimaldi
Mar 31, 2015·European Journal of Pharmacology·Toshihisa Komori
Jun 5, 2016·Journal of Biomechanics·Andrés Julián Arias-MorenoBert van Rietbergen
Oct 28, 2016·Biological Trace Element Research·Shanshan Qi, Hongxing Zheng
Dec 13, 2016·Clinical Chemistry·Matthew B GreenblattMarc N Wein
May 27, 2017·Calcified Tissue International·Xiuhua WuQingan Zhu
Sep 13, 2017·Toxicology and Applied Pharmacology·Yun-Ho HwangSung-Tae Yee

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Citations

May 14, 2019·Expert Opinion on Biological Therapy·Sahar MohsinErnest A Adeghate
Apr 11, 2019·Journal of Bone and Mineral Metabolism·Xiuhua WuQingan Zhu
Jan 8, 2021·Frontiers in Aging Neuroscience·Zhengran YuXuenong Zou
May 1, 2021·International Journal of Molecular Sciences·Antonia DonatJohannes Keller

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