Metformin attenuates motility, contraction, and fibrogenic response of hepatic stellate cells in vivo and in vitro by activating AMP-activated protein kinase

World Journal of Gastroenterology : WJG
Zhen LiChun-Qing Zhang

Abstract

To investigate the effect of metformin on activated hepatic stellate cells (HSCs) and the possible signaling pathways involved. A fibrotic mouse model was generated by intraperitoneal injection of carbon tetrachloride (CCl4) and subsequent treatment with or without metformin. The level of fibrosis was detected by hematoxylin-eosin staining, Sirius Red staining, and immunohistochemistry. The HSC cell line LX-2 was used for in vitro studies. The effect of metformin on cell proliferation (CCK8 assay), motility (scratch test and Transwell assay), contraction (collagen gel contraction assay), extracellular matrix (ECM) secretion (Western blot), and angiogenesis (ELISA and tube formation assay) was investigated. We also analyzed the possible signaling pathways involved by Western blot analysis. Mice developed marked liver fibrosis after intraperitoneal injection with CCl4 for 6 wk. Metformin decreased the activation of HSCs, reduced the deposition of ECM, and inhibited angiogenesis in CCl4-treated mice. Platelet-derived growth factor (PDGF) promoted the fibrogenic response of HSCs in vitro, while metformin inhibited the activation, proliferation, migration, and contraction of HSCs, and reduced the secretion of ECM. Metformin decrease...Continue Reading

References

Feb 24, 2001·Cancer Metastasis Reviews·E BerraJ Pouysségur
Jul 23, 2002·Frontiers in Bioscience : a Journal and Virtual Library·Massimo Pinzani
Aug 16, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Liyan MiaoJohn Zhang
Feb 28, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·June Sung LeeVijay H Shah
Jun 29, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jonel TrebickaJörg Heller
Dec 22, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Alessandra CaligiuriFabio Marra
Jan 23, 2009·Journal of Hepatology·Mercedes FernándezJaume Bosch
Nov 16, 2010·Liver International : Official Journal of the International Association for the Study of the Liver·Stephanie CoulonHans Van Vlierberghe
Jan 22, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Sara AleffiFabio Marra
Apr 19, 2011·Best Practice & Research. Clinical Gastroenterology·Ursula E Lee, Scott L Friedman
Nov 1, 2011·Cardiovascular Research·Daniela CarnevaleGiuseppe Lembo
Dec 7, 2011·Frontiers in Molecular Neuroscience·Jayashree Karar, Amit Maity
Nov 23, 2013·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·R LieglK H Eibl-Lindner
May 2, 2014·Journal of Cellular and Molecular Medicine·Feng ZhangShizhong Zheng
Jun 27, 2014·World Journal of Gastroenterology : WJG·Gülsüm Özlem Elpek
Aug 6, 2014·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Mercedes Fernandez
Mar 20, 2015·Hepatology International·Jordi Gracia-SanchoJaime Bosch
Jul 4, 2015·American Journal of Physiology. Gastrointestinal and Liver Physiology·Dinesh M TripathiJuan Carlos García-Pagán
Nov 9, 2015·Cytokine & Growth Factor Reviews·Erawan Borkham-Kamphorst, Ralf Weiskirchen
Oct 28, 2016·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Guadalupe Garcia-TsaoJaime Bosch
Jul 13, 2015·Cellular and Molecular Gastroenterology and Hepatology·Claudia BoccaMaurizio Parola
Apr 9, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Kerui FanLi Zhang

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Citations

Feb 7, 2019·Journal of Biomedical Materials Research. Part a·Jia HuangWei Liu
Jul 25, 2019·Transplantation·Can-Xiang LinJun-Meng Zheng
Nov 30, 2018·Frontiers in Cell and Developmental Biology·Wei Hou, Wing-Kin Syn
Jan 19, 2020·Nature Reviews. Urology·Julieta AfonsoFátima Baltazar
May 14, 2019·BioMed Research International·Xu LiPujun Gao
Jan 19, 2019·Molecular Cancer Research : MCR·Peter C HartIris L Romero
Dec 23, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Gang WangMao Luo
May 13, 2021·Hepatobiliary & Pancreatic Diseases International : HBPD INT·Devaraj Ezhilarasan
Jun 3, 2021·International Journal of Molecular Sciences·Andrés TabernillaMathieu Vinken
May 20, 2021·Journal of Diabetes Research·Maoyan WuYong Xu
Jun 29, 2021·Biophysics Reviews·Pearlin Hameed, Geetha Manivasagam
Oct 31, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Dongxiao MengChunqing Zhang

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Methods Mentioned

BETA
ELISA
PCR

Software Mentioned

GraphPad Prism
Pro Plus
SPSS19
Image
ImageJ

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