Metformin attenuates triglyceride accumulation in HepG2 cells through decreasing stearyl-coenzyme A desaturase 1 expression

Lipids in Health and Disease
Xiaopeng ZhuXin Gao

Abstract

The prevalence of nonalcoholic fatty liver disease (NAFLD) has increased worldwide. Metformin decreases triglyceride (TG) accumulation in hepatocytes in vivo and in vitro. Stearyl-coenzyme A desaturase 1 (SCD1) knockout mice also show decreased liver TG accumulation; however, whether SCD1 plays a role in the effect of metformin on TG accumulation is unknown. Therefore, the aim of this study was to investigate whether SCD1 mediated the effect of metformin on TG accumulation. HepG2 and AML12 cells were exposed to high glucose and high insulin with or without metformin. An adenovirus was used for the SCD1 knockdown and overexpression. The triglyceride level in cells was detected. The expression of related genes was detected by Western blot and quantitative real-time PCR. A dual-luciferase reporter assay was used to determine the effect of metformin on the transcriptional activity of the SCD1 promoter. Metformin decreased TG accumulation to normal level in HepG2 cells exposed to high glucose and high insulin. The expression of SCD1 and fatty acid synthetase (FAS) was also decreased to normal level by metformin. Knockdown of SCD1 mimicked the effect of metformin on decreasing TG levels in AML12 cells, and the overexpression of SCD1 ...Continue Reading

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Citations

Jan 17, 2019·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Ania RashidpourIsidoro Metón
Sep 10, 2020·International Journal of Food Sciences and Nutrition·Magdalena MikaMaja Grabacka
Feb 19, 2019·International Journal of Endocrinology·Fernanda VacanteIleana Terruzzi
Sep 23, 2018·International Journal of Molecular Sciences·Joseph ZhouLixin Li
Mar 12, 2020·Frontiers in Cardiovascular Medicine·Alan Chait, Laura J den Hartigh
Feb 11, 2021·Pharmaceuticals·Józef Drzewoski, Markolf Hanefeld
Apr 16, 2021·European Journal of Medicinal Chemistry·Deepika KathuriaPrasad V Bharatam
Oct 17, 2020·Environmental Science & Technology·Chun-Yun ZhangHans-Joachim Lehmler
Oct 14, 2021·Cell Death & Disease·Wenfang XiongXiaoping Zou
Nov 19, 2021·Adipocyte·Jingwei ZhangYong Q Chen

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