Metformin Induces Apoptosis and Alters Cellular Responses to Oxidative Stress in Ht29 Colon Cancer Cells: Preliminary Findings

International Journal of Molecular Sciences
Paola SenaLuca Roncucci

Abstract

Accumulating evidence suggests that metformin, used as an antidiabetic drug, possesses anti-cancer properties. Metformin reduced the incidence and growth of experimental tumors in vivo. In a randomized clinical trial among nondiabetic patients, metformin treatment significantly decreased the number of aberrant crypt foci compared to the untreated group with a follow-up of 1 month. In our study, HT29 cells were treated with graded concentrations of metformin, 10 mM/25 mM/50 mM for 24/48 h. We performed immunofluorescence experiments by means of confocal microscopy and western blot analysis to evaluate a panel of factors involved in apoptotic/autophagic processes and oxidative stress response. Moreover, HT29 cells treated with metformin were analyzed by a flow cytometry assay to detect the cell apoptotic rate. The results demonstrate that metformin exerts growth inhibitory effects on cultured HT29 cells by increasing both apoptosis and autophagy; moreover, it affects the survival of cultured cells inhibiting the transcriptional activation of Nuclear factor E2-related factor 2 (NRF-2) and nuclear factor-kappa B (NF-κB). The effects of metformin on HT29 cells were dose- and time-dependent. These results are very intriguing since me...Continue Reading

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Citations

Dec 14, 2019·Journal of Experimental & Clinical Cancer Research : CR·Muhamad Noor Alfarizal KamarudinIshwar Parhar
Apr 19, 2020·Hormone Molecular Biology and Clinical Investigation·Sandro SacchiAntonio La Marca
Aug 23, 2019·International Journal of Molecular Sciences·Simona PompiliGiovanni Latella
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Jul 30, 2021·World Journal of Gastrointestinal Oncology·Hande BeklenKazim Yalcin Arga
Jul 13, 2018·Molecular Pharmaceutics·Yoni DonnerKristen Fortney

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Methods Mentioned

BETA
xenografts
xenograft
confocal microscopy
flow cytometry
nuclear translocation

Software Mentioned

Elements System
NIH Image J
Leica LCS
NIS

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