Metformin prevents nerve growth factor-dependent proliferative and proangiogenic effects in epithelial ovarian cancer cells and endothelial cells

Therapeutic Advances in Medical Oncology
Maritza P GarridoC Romero

Abstract

Epithelial ovarian cancer (EOC) is characterized by exacerbated angiogenesis regulated by proangiogenic and growth factors. Nerve growth factor (NGF) is overexpressed in EOC where it promotes proliferation as well as survival and is considered a proangiogenic factor. Metformin, a drug commonly used in the treatment of diabetes, is attributed to antineoplastic effects, but the underlying mechanisms remain unknown. Given that current therapies yield modest results in EOC patients, the aim of this study was to determine the effects of metformin on NGF-enhanced proliferation of EOC cells and the angiogenic potential of endothelial cells. A2780 (EOC), HOSE (human ovarian surface epithelial) and EA.hy926 (endothelial) cells were treated with NGF and metformin. Cell viability, cell proliferation and cell cycle were evaluated in all three cell lines, and the angiogenic potential in endothelial EA.hy926 cells. NGF enhanced cell proliferation in A2780, HOSE and EA.hy926 cells (p < 0.05), while metformin treatment decreased cell proliferation in A2780 and EA.hy926 cells (p < 0.05). Moreover, the NGF-enhanced angiogenic score in EA.hy926 cells was prevented by metformin (p < 0.05). Given that NGF plays a significant role in EOC progression...Continue Reading

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Citations

Oct 15, 2019·Frontiers in Oncology·Maritza P GarridoCarmen Romero
Oct 22, 2020·Pharmaceuticals·Maritza P GarridoCarmen Romero
Oct 22, 2020·International Journal of Molecular Sciences·Maritza P GarridoCarmen Romero
Apr 3, 2021·SAGE Open Medicine·Yu Ren, Hua Luo
Aug 20, 2021·Saudi Pharmaceutical Journal : SPJ : the Official Publication of the Saudi Pharmaceutical Society·Nezar BoreakShankargouda Patil
Nov 11, 2021·Journal of Drug Targeting·Bartłomiej BarczyńskiJan Kotarski

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Methods Mentioned

BETA
FACS
flow cytometry
FCS

Software Mentioned

Fiji ImageJ
ImageJ
Image Pro Plus

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