Methamphetamine cytotoxicity and effect on LPS-stimulated IL-1beta production by human monocytes

Toxicology in Vitro : an International Journal Published in Association with BIBRA
D A TiptonM Kh Dabbous

Abstract

Methamphetamine (METH) abuse is associated with "METH mouth", characterized by rampant dental decay and destruction of periodontal bone and soft tissues. In periodontitis, monocyte/macrophages, stimulated by bacterial lipopolysaccharide (LPS), produce interleukin-1beta (IL-1beta), contributing to bone and soft tissue degradation. Effects of METH on monocyte/macrophages and its role in periodontitis are unknown. The objective of this study was to determine METH cytotoxicity and effects on constitutive and LPS-stimulated IL-1beta production in THP-1 human monocytes. METH significantly reduced cell viability, assessed by activity of a mitochondrial enzyme, by 20-40% after 24h, with recovery at longer periods. Brief exposure to METH caused <10% cytotoxicity (measured by an assay that detects membrane damage). LPS from E. coli or the periodontopathogen Fusobacterium nucleatum (F. n.) significantly increased IL-1beta production (measured by ELISA). Despite cytotoxicity of some METH concentrations, METH had no significant effect on constitutive IL-1beta production. However, METH generally increased LPS-stimulated IL-1beta levels, reaching statistical significance at 5x10(-5)M METH ( approximately 50% to >100% increase). The study sugg...Continue Reading

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Citations

Jun 13, 2012·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Jessica L ReynoldsStanley A Schwartz
Jan 30, 2015·Journal of Prosthodontics : Official Journal of the American College of Prosthodontists·Meredith A GantosCortino Sukotjo
Jul 16, 2015·Clinical Oral Investigations·Niklas RommelMarco R Kesting
Feb 22, 2011·Brain, Behavior, and Immunity·Jonathan P WisorWilliam C Clegern
Sep 11, 2018·Periodontology 2000·Francis J Hughes, P Mark Bartold

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