Methamphetamine Increases the Proportion of SIV-Infected Microglia/Macrophages, Alters Metabolic Pathways, and Elevates Cell Death Pathways: A Single-Cell Analysis.

Viruses
Meng NiuHoward S Fox

Abstract

Both substance use disorder and HIV infection continue to affect many individuals. Both have untoward effects on the brain, and the two conditions often co-exist. In the brain, macrophages and microglia are infectable by HIV, and these cells are also targets for the effects of drugs of abuse, such as the psychostimulant methamphetamine. To determine the interaction of HIV and methamphetamine, we isolated microglia and brain macrophages from SIV-infected rhesus monkeys that were treated with or without methamphetamine. Cells were subjected to single-cell RNA sequencing and results were analyzed by statistical and bioinformatic analysis. In the animals treated with methamphetamine, a significantly increased proportion of the microglia and/or macrophages were infected by SIV. In addition, gene encoding functions in cell death pathways were increased, and the brain-derived neurotropic factor pathway was inhibited. The gene expression patterns in infected cells did not cluster separately from uninfected cells, but clusters comprised of microglia and/or macrophages from methamphetamine-treated animals differed in neuroinflammatory and metabolic pathways from those comprised of cells from untreated animals. Methamphetamine increases C...Continue Reading

References

May 23, 1998·Neurochemistry International·J L Cadet, C Brannock
Oct 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·M C MarcondesH S Fox
May 22, 2003·The American Journal of Pathology·Eleanor S RobertsHoward S Fox
Dec 6, 2003·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Dianne LangfordUNKNOWN HIV Neurobehavioral Research Center Group
Jan 31, 2004·Journal of the International Neuropsychological Society : JINS·Julie D RippethUNKNOWN HNRC Group
May 13, 2004·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Birgit A VöllmPaul M Matthews
May 28, 2004·The Journal of Pharmacology and Experimental Therapeutics·David M ThomasDonald M Kuhn
Dec 31, 2004·Journal of Neuropathology and Experimental Neurology·Eleanor S RobertsHoward S Fox
Mar 2, 2005·Journal of Urban Health : Bulletin of the New York Academy of Medicine·Elizabeth LambertDavid Vlahov
Mar 4, 2006·AIDS·Beryl A KoblinSusan Buchbinder
Nov 18, 2006·Journal of Urban Health : Bulletin of the New York Academy of Medicine·Steven Shoptaw, Cathy J Reback
Feb 28, 2007·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Michael W PlankeyLisa P Jacobson
Apr 17, 2007·European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology·Francesco AngelucciAleksander A Mathé
Dec 7, 2007·Proceedings of the National Academy of Sciences of the United States of America·A RosaI Bozzoni
May 7, 2008·The American Journal of Pathology·Hao LiangWen-Zhe Ho
May 30, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yoshimoto SekineJean L Cadet
Jul 12, 2008·The Journal of Infectious Diseases·Tricia H BurdoHoward S Fox
Nov 10, 2009·AIDS Research and Human Retroviruses·Sima Shelly ToussiHarris Goldstein
Nov 10, 2009·International Review of Neurobiology·Jean Lud Cadet, Irina N Krasnova
Dec 10, 2009·AIDS·Samanta SimioniRenaud A Du Pasquier
May 22, 2010·The American Journal of Pathology·Maria Cecilia Garibaldi MarcondesHoward S Fox
Jul 8, 2010·The International Journal of Neuropsychopharmacology·Anders B KleinSusana Aznar
Aug 4, 2011·Archives of Pediatrics & Adolescent Medicine·Peter FreemanUNKNOWN Adolescent Trials Network for HIV/AIDS Interventions 016b Team
Sep 29, 2011·Nature Neuroscience·Marco PrinzRichard M Ransohoff
Nov 26, 2011·Neurobiology of Disease·S Ares-SantosR Moratalla
Jun 21, 2012·Neurotoxicity Research·Kenneth H ClarkCharles W Bradberry
Oct 20, 2012·AIDS·Fabrice BonnetUNKNOWN S CO3 Aquitaine Cohort
Dec 21, 2012·Nature Reviews. Neuroscience·Hyungju Park, Mu-ming Poo
Sep 3, 2013·Neuroscience Letters·Danielle M Friend, Kristen A Keefe
Nov 28, 2013·Behavioral Medicine·Ramani Durvasula, Theodore R Miller
Dec 3, 2013·Acta Neuropathologica·Jean Lud CadetChristopher Mark Milroy
Dec 18, 2013·Bioinformatics·Andreas KrämerStuart Tugendreich

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE160384

Methods Mentioned

BETA
scRNA-seq
DNA assay
Assay
Chip
PCA

Software Mentioned

10x
Flow
IPA
UMAP
SIVE
CHARTER
Ingenuity Pathway Analysis ( IPA )
Partek
Genomics Cellranger
Biomart

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.