PMID: 11317884Apr 25, 2001Paper

Methane microprofiles in a sewage biofilm determined with a microscale biosensor

Water Research
L R DamgaardN P Revsbech

Abstract

Microprofiles of the methane concentration in a 3.5-mm-thick sewage outlet biofilm were measured at high spatial and temporal resolution using a microscale biosensor for methane. In the freshly collected biofilm, methane was building up to a concentration of 175 mumol l-1 at 3 mm depth with a total methanogenesis of 0.14 mumol m-2 s-1, as compared to an aerobic respiration (including methane oxidation) of 0.80 mumol m-2 s-1. A model biofilm was established by homogenisation of an in situ biofilm and 12 days of incubation with surplus sodium acetate. The homogenised biofilm was able to maintain 50% of the methanogenic activity in the absence of external electron donor. Oxygen had only a minor effect on the methane production, but aerobic respiration consumed a substantial part of the produced methane and was thus an important control on methane export from the biofilm. A concentration of 2 mmol l-1 nitrate was shown to inhibit methanogenesis only in the upper layer of the biofilm, whereas a further addition of 2 mmol l-1 sulphate inhibited methanogenesis in the entire biofilm. The study demonstrated the power of the methane microsensor in the study of microhabitats with concurrent production and consumption of methane.

References

Sep 1, 1997·Analytical Chemistry·L H LarsenN P Revsbech

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Citations

Feb 12, 2008·Nature Reviews. Microbiology·Philip S Stewart, Michael J Franklin
May 10, 2002·Environmental Microbiology·Neil D GrayIan M Head
Mar 29, 2006·Journal of Burn Care & Research : Official Publication of the American Burn Association·Ariel U SpencerLawrence J Gottlieb
Oct 2, 2007·Applied and Environmental Microbiology·Hisashi SatohSatoshi Okabe
Jun 17, 2008·Applied and Environmental Microbiology·V R Phoenix, W M Holmes
Apr 19, 2015·The Science of the Total Environment·Yiwen LiuZhiguo Yuan
Mar 21, 2009·Journal of Applied Microbiology·C G ZhaoM M F Choi
Apr 6, 2006·Biotechnology and Bioengineering·Claudio Garibay-OrijelHéctor Mario Poggi-Varaldo
Feb 28, 2015·Environmental Science & Technology·P J StrongW P Clarke
Dec 24, 2010·Journal of Hazardous Materials·F A BatziasP-M P Spanidis
Jun 21, 2016·Biotechnology & Genetic Engineering Reviews·Neelam MangwaniSurajit Das
Sep 7, 2014·Applied and Environmental Microbiology·Jing SunZhiguo Yuan
Dec 5, 2018·Environmental Science and Pollution Research International·Rita Ventura MatosJosé Saldanha Matos

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