Methanobacterium kanagiense sp. nov., a hydrogenotrophic methanogen, isolated from rice-field soil

International Journal of Systematic and Evolutionary Microbiology
Koji KitamuraAkio Tonouchi

Abstract

A pure culture of an obligately anaerobic, hydrogenotrophic, methanogenic archaeon, designated strain 169(T), which grows with hydrogen and carbon dioxide as the sole energy and carbon sources, was isolated from an anaerobic propionate-oxidizing enrichment culture originally obtained as an inoculant from rice-field soil in Japan. Cells of strain 169(T) were non-motile, Gram-reaction-variable and rod-shaped or slightly curved rods with rounded ends (1.6-5.0 × 0.35-0.5 µm). Strain 169(T) had fimbriae at both ends of the cell (up to ~10 per cell) but did not possess flagella. Ultrathin sections showed a single-layered, electron-dense cell wall about 6 nm thick, which is typical of Gram-positive bacteria. Growth was observed at 15 °C-45 °C (optimum 40 °C), at pH  6.5-9.6 (optimum pH 7.5-8.5) and in 0-70 g NaCl l(-1) (0-1.2 M) (optimum 5 g NaCl l(-1); 0.086 M). Strain 169(T) utilized only hydrogen and carbon dioxide as energy and carbon sources. The DNA G+C content was 39.3 mol%. The results of 16S rRNA gene sequence analysis indicated that strain 169(T) was most closely related to Methanobacterium subterraneum DSM 11074(T) (96.8 % sequence similarity) and Methanobacterium formicicum DSM 1535(T) (96.4 %). On the basis of its morphol...Continue Reading

References

Mar 1, 1968·Journal of Bacteriology·K F LangenbergR S Wolfe
Jul 20, 2004·Proceedings of the National Academy of Sciences of the United States of America·Koichiro TamuraSudhir Kumar
Apr 1, 1989·Plant Physiology·S J SungC C Black
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar

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Citations

Jun 26, 2012·Applied and Environmental Microbiology·Myron T La DucKasthuri Venkateswaran
Feb 22, 2014·International Journal of Systematic and Evolutionary Microbiology·Akio TonouchiTakashi Fujita
Apr 30, 2013·International Journal of Systematic and Evolutionary Microbiology·Takuya HondaAkio Tonouchi
Apr 24, 2012·International Journal of Systematic and Evolutionary Microbiology·Akio TonouchiTakashi Fujita
Jan 23, 2014·International Journal of Systematic and Evolutionary Microbiology·Hinsby Cadillo-QuirozStephen H Zinder
Sep 6, 2011·International Journal of Systematic and Evolutionary Microbiology·Guillaume BorrelGérard Fonty
Apr 22, 2017·The Science of the Total Environment·Singh AlpanaS K Dubey
Jan 12, 2017·Archaea : an International Microbiological Journal·Ayrat M ZiganshinMarcell Nikolausz
Aug 20, 2019·Applied and Environmental Microbiology·Michiel H In 't ZandtCornelia U Welte
Nov 25, 2020·The Science of the Total Environment·Farrukh Raza AminRuihong Zhang
Dec 2, 2020·The Science of the Total Environment·Yanli GuoShaohui Zhang
Dec 4, 2020·Biotechnology Advances·Kevin PfeiferSimon K-M R Rittmann

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