Methanobrevibacter smithii Archaemia in Febrile Patients With Bacteremia, Including Those With Endocarditis.

Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America
Michel DrancourtDidier Raoult

Abstract

The spectrum of infections caused by methanogens remains to be described. We searched for methanogens in the blood of febrile patients using specific tools. Blood culture samples routinely collected in patients with fever were prospectively screened by specific PCR assays for methanogens. Positive samples were observed by autofluorescence and electron microscopy, analyzed by metagenomics and cultured using previously developed methods. Blood culture bottles experimentally inoculated were used as controls. The presence of methanogens in vascular and cardiac tissues was assessed by indirect immunofluorescence, fluorescent in situ hybridization and PCR-based investigations. PCR detection attempted in 7,716 blood samples, was negative in all 1,312 aerobic bottles and 810 bacterial culture-negative anaerobic bottles. PCRs were positive in 27/5,594 (0.5%) bacterial culture-positive anaerobic bottles collected from 26 patients. Sequencing confirmed Methanobrevibacter smithii associated with staphylococci in 14 patients, Enterobacteriaceae in nine patients and streptococci in three patients. Metagenomics confirmed M. smithii in five samples, and M. smithii was isolated in broth from two samples; the genomes of these two isolates were s...Continue Reading

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Dec 11, 2019·Scientific Reports·Amadou Hamidou TogoDidier Raoult

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Citations

Dec 31, 2020·Microorganisms·Cheick Oumar GuindoGhiles Grine
Jun 26, 2021·Microbial Ecology·Kenza DjemaiMaryam Tidjani Alou
Jun 19, 2021·New Microbes and New Infections·K DjemaiG Grine

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