Methanobrevibacter smithii is the predominant methanogen in patients with constipation-predominant IBS and methane on breath.

Digestive Diseases and Sciences
Gene KimM Pimentel

Abstract

Among irritable bowel syndrome (IBS) patients, breath methane producers overwhelmingly have constipation predominance (C-IBS). Although the most common methanogen in humans is Methanobrevibacter smithii, incidence and type of methanogenic bacteria in C-IBS patients are unknown. By use of a questionnaire and lactulose breath testing, subjects with Rome II C-IBS and methane (>3 ppm) were selected (n = 9). The control group included subjects with IBS who had no breath methane (n = 10). Presence of bacterial DNA was assessed in a stool sample of each subject by quantitative-PCR using universal 16S rDNA primer. M. smithii was quantified by use of a specific rpoB gene primer. M. smithii was detected in both methane and non-methane subjects. However, counts and relative proportion of M. smithii were significantly higher for methane-positive than for methane-negative subjects (1.8 × 10(7) ± 3.0 × 10(7) vs 3.2 × 10(5) ± 7.6 × 10(5) copies/g wet stool, P < 0.001; and 7.1 ± 6.3 % vs 0.24 ± 0.47 %, P = 0.02 respectively). The minimum threshold of M. smithii resulting in positive lactulose breath testing for methane was 4.2 × 10(5) copies/g wet stool or 1.2 % of total stool bacteria. Finally, area-under-curve for breath methane correlated s...Continue Reading

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Citations

Oct 24, 2013·Current Gastroenterology Reports·Mark PimentelChristopher Chang
May 3, 2014·Digestive Diseases and Sciences·Mark PimentelMeridythe Amichai
Apr 23, 2014·Nature Reviews. Gastroenterology & Hepatology·Stephen M Collins
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Jul 15, 2015·Expert Review of Gastroenterology & Hepatology·Magdy El-Salhy
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Feb 6, 2020·The American Journal of Gastroenterology·Mark PimentelSatish S C Rao

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