Methanogenic conversion of 3-s-methylmercaptopropionate to 3-mercaptopropionate.

Applied and Environmental Microbiology
M J van der MaarelT A Hansen

Abstract

Anaerobic metabolism of dimethylsulfoniopropionate, an osmolyte of marine algae, in anoxic intertidal sediments involves either cleavage to dimethylsulfide or demethylation to 3-S-methylmercaptopropionate (MMPA) and subsequently to 3-mercaptopropionate. The methanogenic archaea Methanosarcina sp. strain MTP4 (DSM 6636), Methanosarcina acetivorans DSM 2834, and Methanosarcina (Methanolobus) siciliae DSM 3028 were found to use MMPA as a growth substrate and to convert it stoichiometrically to 3-mercaptopropionate. Approximately 0.75 mol of methane was formed per mol of MMPA degraded; methanethiol was not detected as an intermediate. Eight other methanogenic strains did not carry out this conversion. We also studied the conversion of MMPA in anoxic marine sediment slurries. Addition of MMPA (500 (mu)M) resulted in the production of methanethiol which was subsequently converted to methane (417 (mu)M). In the presence of the antibiotics ampicillin, vancomycin, and kanamycin (20 (mu)g/ml each), 275 (mu)M methane was formed from 380 (mu)M MMPA; no methanethiol was formed during these incubations. Only methanethiol was formed from MMPA when 2-bromoethanesulfonate (25 mM) was added to a sediment suspension. These results indicate that i...Continue Reading

References

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Citations

Jan 3, 2001·Applied and Environmental Microbiology·M Jansen, T A Hansen
Sep 8, 2009·Molecular Microbiology·Arpita BoseWilliam W Metcalf
Feb 19, 2015·Journal of Bacteriology·He Fu, William W Metcalf
Nov 22, 2015·Applied and Environmental Microbiology·Leonie WenningAlexander Steinbüchel
Jun 28, 2018·Physical Chemistry Chemical Physics : PCCP·Samantha SengYeny A Tobon
Nov 1, 1996·Applied and Environmental Microbiology·M J van der MaarelT A Hansen
Sep 1, 1996·Applied and Environmental Microbiology·R Boopathy

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