Methanogenic potential of formate in thermophilic anaerobic digestion

Waste Management & Research : the Journal of the International Solid Wastes and Public Cleansing Association, ISWA
Philipp LinsPaul Illmer

Abstract

In the present study the methanogenic potential of formate (HCOO(-)) during thermophilic anaerobic digestion was investigated. After appropriate conditions for methanogenesis (HCOO(-) and inoculum concentration, pH and duration of incubation) were assessed, an experiment with initial 31 replicates was run. Diluted fermenter sludge was used as inoculum, and process parameters including the pH, quality and quantity of the produced biogas and the concentrations of volatile fatty acids and HCO(3) (-) were determined. Remarkably, after 5 days of incubation the highest CH(4) production was calculated for a HCOO(-) concentration of 200 mmol L(-1), a concentration, however, which might not occur in situ. During the phase of high CH(4) production HCOO(-) was degraded with a rate of 1.5 mmol L(-1) h(-1), and distinct changes of Gibbs free energy for several reactions were observed. Based on denaturing high-performance liquid chromatography, denaturing gradient gel electrophoresis, and additional subsequent sequencing approaches the hydrogenotrophic Methanothermobacter wolfeii was the dominant methanogen responsible for CH(4) production. Further confirmation was achieved due to the detection of autofluorescing rods with a size of up to ~3...Continue Reading

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Citations

Mar 13, 2014·Journal of Industrial Microbiology & Biotechnology·Ru WangLan Wang
Apr 20, 2014·Waste Management & Research : the Journal of the International Solid Wastes and Public Cleansing Association, ISWA·Héctor M Poggi-VaraldoNoemí F Rinderknecht-Seijas
Jul 20, 2018·Waste Management & Research : the Journal of the International Solid Wastes and Public Cleansing Association, ISWA·Sophia GhanimehElsy Ibrahim
Oct 5, 2019·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Anna DolomanCharles D Miller
Sep 14, 2016·Engineering in Life Sciences·Andreas O WagnerChristian Ebner
Aug 28, 2020·Environmental Science & Technology·Miao YanIrini Angelidaki

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Methods Mentioned

BETA
PCR
electrophoresis

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