Methanol/sorbitol co-feeding induction enhanced porcine interferon-α production by P. pastoris associated with energy metabolism shift

Bioprocess and Biosystems Engineering
Min-Jie GaoChi-Chung Lin

Abstract

The production of porcine interferon-α (pIFN-α) by Pichia pastoris was largely enhanced when adopting sorbitol/methanol co-feeding induction strategy at 30 °C in a 10-L fermentor. Analysis of energy regeneration pattern and carbon metabolism revealed that major energy metabolism energizing pIFN-α synthesis shifted from formaldehyde dissimilatory energy metabolism pathway to TCA cycle under the methanol/sorbitol co-feeding induction strategy. The sorbitol/methanol co-feeding induction strategy weakened formaldehyde dissimilatory pathway and repressed the accumulation of toxic metabolite-formaldehyde, reduced theoretical oxygen consumption rate and oxygen supply requirement, and increased energy/methanol utilization efficiency so that more methanol could be effectively used for pIFN-α synthesis. As a result, pIFN-α antiviral activity reached a highest level of 1.8 × 10(7) IU/mL which was about 10- to 200-folds of those obtained under pure methanol induction at 20 and 30 °C, respectively.

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Citations

Nov 5, 2014·Journal of Industrial Microbiology & Biotechnology·Rongrong ZhanTao Zhang
Feb 15, 2020·Journal of Cellular Physiology·Mohsen KarbalaeiHadi Farsiani
Sep 2, 2020·The Analyst·Mayur ParekhChristopher Lennon
Jun 2, 2018·Research in Pharmaceutical Sciences·Saeed AzadiRoya Solaimanian

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