Methanosarcina flavescens sp. nov., a methanogenic archaeon isolated from a full-scale anaerobic digester

International Journal of Systematic and Evolutionary Microbiology
Tobias KernMichael Rother

Abstract

A novel, strictly anaerobic, methanogenic archaeon, strain E03.2, was isolated from a full-scale biogas plant in Germany. Cells were non-motile sarcina-like cocci, occurring in aggregates. Strain E03.2 grows autotrophically on H2 plus CO2, additionally cells can utilize acetate, methanol, moni-, di- and trimethylamine as carbon and energy sources; however, growth or methanogenesis on formate was not observed. Yeast extract and vitamins stimulate growth but were not mandatory.The optimal growth temperature was approximately 45 °C; maximal growth rates were obtained at pH about 7.0 at approximately 6.8 mM NaCl. The DNA G+C content of strain E03.2T was 41.3 mol%. Phylogenetic analyses based on 16S rRNA and mcrA gene sequences placed strain E03.2T within the genus Methanosarcina. Based on 16S rRNA gene sequence identity strain E03.2T was related to seven different Methanosarcina species, but most closely related to M. thermophila TM-1T. Phenotypical, physiological and genomic characteristics indicated that strain E03.2T represents a novel species. The name Methanosarcina flavescens sp. nov. is proposed for this novel species, with strain E03.2T (=DSM 100822 =JCM 30921) as the type strain.

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Citations

Jan 6, 2018·AMB Express·Franziska EnzmannDirk Holtmann
Feb 24, 2019·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·E SogodogoGhiles Grine
Sep 27, 2019·Frontiers in Microbiology·Martin A FischerRuth A Schmitz
Jun 5, 2020·Acta Crystallographica. Section D, Structural Biology·P N BlankJ A Himmelberger
Dec 12, 2020·Biotechnology and Bioengineering·Aaron LeiningerBirthe Kjellerup
Nov 25, 2020·The Science of the Total Environment·Farrukh Raza AminRuihong Zhang
May 2, 2021·Applied and Environmental Microbiology·Jinjie ZhouDerek R Lovley

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