PMID: 9450520Feb 5, 1998Paper

Methional, a cellular metabolite, induces apoptosis preferentially in G2/M-synchronized BAF3 murine lymphoid cells

Cytometry
A M RochG Quash

Abstract

We have previously shown that methional, derived from 4-methylthio-2-oxobutanoate, is a cellular mediator of apoptosis in BAF3 b0 murine lymphoid cells, which are dependent on IL3 for their growth in culture. When cells synchronized in S phase by double thymidine block were treated with methional immediately after thymidine withdrawal, methional was unable to induce DNA-strand breaks, whereas it inhibited the progression of cells from S to G2/M phases. This inhibition of cell cycle progression was associated with a 53% decrease in DNA synthesis. In contrast, when BAF3 b0 cells were synchronized in G2/M phase using SK&F 96365, and treated with methional immediately after drug removal, methional induced DNA-strand breaks in 49% of cells in 4 h, compared to 12% in controls. As contact time increased from 4 to 8 h, DNA-strand breaks increased to 94% in methional-treated cells compared to 11% in controls. These observations on G2/M-synchronized cells are different from those seen in BAF3b0 cells in G1 phase, 3 h after their release from the G2/M block, in that there was no decrease in size of the G1 population even after an additional 4 h incubation in the presence of methional. These results, taken together, provide a rational basi...Continue Reading

Citations

Jan 29, 2003·Environmental and Molecular Mutagenesis·Sophie MeintièresDaniel Marzin
Aug 9, 2003·Biochemical Pharmacology·Gerard QuashUwe Reichert
Jul 13, 2002·Annals of the New York Academy of Sciences·Maurizio CutoloBarbara Villaggio
Oct 9, 2019·Environmental Science and Pollution Research International·Jaroslav SemerádTomáš Cajthaml

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis