Methionine acts as a "magnet" in photoaffinity crosslinking experiments

FEBS Letters
Angela WittelsbergerMichael Rosenblatt

Abstract

Photoaffinity crosslinking has been utilized to probe the nature of the ligand-receptor interface for a number of G protein-coupled receptor systems. Often the photoreactive benzophenone moiety incorporated in the ligand is found to react with a methionine in the receptor. We introduced methionines one-at-a-time into the region 163-176 of the parathyroid hormone receptor, and find that crosslinking occurs to the side-chain of methionine over a range of 11 amino acids. We call this the "Magnet Effect" of methionine. Hence, crosslinking contact points can be significantly shifted by the presence of methionine in a receptor domain.

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May 8, 2008·Biochemistry·Paul MonaghanMichael Rosenblatt
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