Methionine and choline supply during the peripartal period alter polymorphonuclear leukocyte immune response and immunometabolic gene expression in Holstein cows

Journal of Dairy Science
Zheng ZhouJ J Loor

Abstract

Polymorphonuclear leukocytes (PMNL) are the first responders upon pathogen invasion and hence play an important role in inflammatory and immune responses. Rumen-protected methionine (MET) and choline (CHOL) during the peripartal period affect the immune response and inflammatory status in dairy cows to different extents. We aimed to examine the effect of MET and CHOL supply on expression of genes regulating key PMNL functions and associations with whole-blood immune challenge. Thirty multiparous Holstein cows from a larger cohort randomly assigned from -21 to 30 d relative to parturition to a basal control (CON) diet, CON plus MET at a rate of 0.08% of dry matter, or CON plus CHOL at 60 g/d were used. Blood was sampled at -10, 7, and 30 d relative to parturition for inflammatory biomarker analyses and PMNL isolation. Neutrophil and monocyte phagocytosis and oxidative burst in vitro were assessed in whole blood at 1, 7, and 28 d. Although neutrophil and monocyte phagocytosis did not differ, oxidative burst in neutrophils and monocytes was greater in MET-supplemented cows relative to CON cows. Compared with CON, PMNL adhesion and migration-related genes (ITGAM, ITGB2, ITGA4) were downregulated in response to MET and CHOL. Express...Continue Reading

References

Jun 1, 1979·British Journal of Haematology·S P SpielbergJ D Schulman
Jun 20, 1986·Journal of Chromatography·Z DeylM Horakova
Sep 1, 1971·Bulletin of Environmental Contamination and Toxicology·P G DymentJ S Wiseman
Jul 30, 1970·The New England Journal of Medicine·B HolmesR A Good
Dec 1, 1980·Agents and Actions·D RoosA A Voetman
Jun 28, 1996·Journal of Chromatography. B, Biomedical Applications·D Fekkes
Oct 1, 1996·FEMS Immunology and Medical Microbiology·L L LaichalkT J Standiford
Jul 10, 1999·Advances in Immunology·M A Cassatella
Nov 22, 2001·The European Journal of Surgery = Acta Chirurgica·B M EganD J Bouchier-Hayes
Apr 24, 2002·Antioxidants & Redox Signaling·Vuokko L KinnulaPirjo Koistinen
May 17, 2006·The Journal of Nutrition·John T Brosnan, Margaret E Brosnan
Nov 26, 2008·Veterinary Immunology and Immunopathology·Lorraine M Sordillo, Stacey L Aitken
Jul 12, 2011·Current Opinion in Pharmacology·Lynne R PrinceLisa C Parker
Dec 27, 2011·Research in Veterinary Science·E TrevisiG Bertoni
Nov 7, 2014·Frontiers in Immunology·Cristina TecchioMarco A Cassatella
Jan 1, 2013·Annual Review of Animal Biosciences·Juan J LoorJames K Drackley
Mar 13, 2015·PLoS Pathogens·Philipp KrugerDominik Hartl
Aug 26, 2016·Immunological Reviews·Jamel El-BennaPham My-Chan Dang

❮ Previous
Next ❯

Citations

Apr 13, 2020·Journal of Dairy Science·J W McFaddenH M White
Aug 19, 2020·Journal of Animal Science·Danielle N ColemanJuan J Loor
Aug 31, 2020·Journal of Animal Science and Biotechnology·Vincenzo LopreiatoErminio Trevisi
May 7, 2020·Frontiers in Veterinary Science·Shuaichen LiJiantao Zhang
Feb 26, 2021·Arteriosclerosis, Thrombosis, and Vascular Biology·Zhan HuJiangping Song

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.