Abstract
During the bladder filling phase, the volume of the urinary bladder increases dramatically, with only minimal increases in intravesical pressure. To accomplish this, the smooth muscle of the bladder wall must remain relaxed during bladder filling. However, the mechanisms responsible for the stabilization of bladder excitability during stretch are unclear. We hypothesized that stretch-dependent K(+) (TREK) channels in bladder smooth muscle cells may inhibit contraction in response to stretch. Bladder tissues from mouse, guinea pig and monkey were used for molecular, patch clamp, mechanical, electrical, Ca(2+) imaging and cystometric responses to methionine and its derivatives, which are putative blockers of stretch-dependent K(+) (SDK) channels. SDK channels are functionally expressed in bladder myocytes. The single channel conductance of SDK channels is 89pS in symmetrical K(+) conditions and is blocked by L-methionine. Expressed TREK-1 currents are also inhibited by L-methioninol. All three types of bladder smooth muscle cells from mouse, guinea pig and monkey expressed TREK-1 genes. L-methionine, methioninol and methionine methyl ester but not D-methionine increased contractility in concentration-dependent manner. Methioninol...Continue Reading
References
Dec 1, 1992·The Journal of General Physiology·D Kim
Dec 1, 1985·Pflügers Archiv : European journal of physiology·U Klöckner, G Isenberg
Aug 1, 1986·The Journal of Urology·R M LevinA J Wein
Dec 1, 1987·British Journal of Urology·R B Kinder, A R Mundy
Feb 1, 1994·British Journal of Urology·C R Chapple, D Smith
Jul 1, 1994·British Journal of Urology·A F Brading
Apr 15, 1995·The Journal of Physiology·R W OrdwayJ J Singer
Dec 20, 1993·Annals of the New York Academy of Sciences·M YoshinoH Yabu
Jul 1, 1997·The American Journal of Physiology·T J HeppnerM T Nelson
Aug 1, 1997·Pharmacology & Therapeutics·W H Turner, A F Brading
Oct 1, 1998·The American Journal of Physiology·G M DickK M Sanders
Sep 10, 1999·The Journal of Biological Chemistry·F MaingretE Honoré
Sep 29, 1999·Gastroenterology·G FarrugiaJ L Rae
Apr 1, 2000·British Journal of Pharmacology·T Weidelt, G Isenberg
Apr 15, 2000·The Journal of Physiology·H HashitaniG D Hirst
Jul 18, 2000·American Journal of Physiology. Cell Physiology·O BayguinovK M Sanders
May 15, 2001·The Journal of Physiology·S D Koh, K M Sanders
Sep 19, 2001·The Journal of Biological Chemistry·S D KohB Horowitz
May 30, 2002·The Journal of General Physiology·Guangju JiMichael I Kotlikoff
Jun 4, 2002·The Journal of Physiology·Gerald M Herrera, Mark T Nelson
Aug 16, 2002·The Journal of Physiology·Grant W HennigTerence K Smith
Jun 19, 2003·The Journal of Physiology·Gerald M HerreraMark T Nelson
Feb 9, 2005·British Journal of Pharmacology·Kyu Joo ParkSang Don Koh
Citations
Dec 14, 2011·Nature Reviews. Urology·Georgi V Petkov
Apr 23, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jun-Ho La, G F Gebhart
Sep 3, 2010·PloS One·Iain L O BuxtonJennifer N Tichenor
Nov 26, 2009·Experimental Biology and Medicine·Eric E LloydRobert M Bryan
Oct 2, 2015·Journal of Neurogastroenterology and Motility·Miroslav RadulovicBing Gong
Sep 4, 2015·Annals of the New York Academy of Sciences·Stephen G Brohawn
Aug 11, 2011·Channels·Jacques NoëlFlorian Lesage
Feb 15, 2014·ISRN Pharmacology·Pradeep TyagiNaoki Yoshimura
Dec 10, 2014·PloS One·Tayyba Y AliRaheela N Khan
May 26, 2017·American Journal of Physiology. Renal Physiology·Ricardo H PinedaAnna P Malykhina
Dec 22, 2009·The Journal of Urology·Salah A BakerSang Don Koh
Oct 24, 2018·Neurourology and Urodynamics·Balachandar NedumaranAnna P Malykhina
Jan 10, 2014·Naunyn-Schmiedeberg's Archives of Pharmacology·Li LuXiaoliang Wang
Mar 1, 2012·Lower Urinary Tract Symptoms·En MengYao-Chi Chuang
Jun 20, 2012·International Journal of Urology : Official Journal of the Japanese Urological Association·Xilian WuWeijie Liang
Apr 17, 2013·British Journal of Pharmacology·U A AndersonK D McCloskey
May 8, 2014·The Journal of Physiology·Qi LeiAnna P Malykhina
Apr 16, 2010·Physiological Reviews·Péter Enyedi, Gábor Czirják
Apr 3, 2013·Endocrinology·Nobuyuki NishikawaOsamu Ogawa
Jul 7, 2020·American Journal of Physiology. Renal Physiology·John Malysz, Georgi V Petkov
May 29, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·V GilM Jiménez
Jun 26, 2012·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Sang Don KohK M Sanders
Jan 13, 2011·The Journal of Physiology·Kevin MonaghanSang Don Koh
Jun 28, 2013·American Journal of Physiology. Cell Physiology·Nathanael S HeymanIain L O Buxton
May 23, 2019·BMC Urology·Ricardo H PinedaAnna P Malykhina
May 23, 2020·International Urogynecology Journal·Şükrü YildizMurat Ekin
Jun 20, 2020·Progress in Biophysics and Molecular Biology·Felix WiedmannConstanze Schmidt
Sep 29, 2020·Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology·Pierluigi MarianiLuigi Laino
Nov 27, 2009·Autonomic Neuroscience : Basic & Clinical·C H FryJ S Young