Methionine-dependence and combination chemotherapy on human gastric cancer cells in vitro

World Journal of Gastroenterology : WJG
Wei-Xin CaoYan-Zhen Lin

Abstract

To elucidate whether human primary gastric cancer and gastric mucosa epithelial cells in vitro can grow normally in a methionine (Met) depleted environment, i.e. Met-dependence, and whether Met-depleting status can enhance the killing effect of chemotherapy on gastric cancer cells. Fresh human gastric cancer and mucosal tissues were managed to form monocellular suspensions, which were then cultured in the Met-free but homocysteine-containing (Met(-)Hcy(+)) medium, with different chemotherapeutic drugs. The proliferation of the cells was examined by cell counter, flow cytometry (FCM) and microcytotoxicity assay (MTT). The growth of human primary gastric cancer cells in Met(-)Hcy(+) was suppressed, manifested by the decrease of total cell counts [1.46 +/- 0.42 (x 10(9).L(-1)) in Met(-)Hcy(+) vs 1.64 +/-0.44(x 10(9).L(-1)) in Met(+)Hcy(-), P<0.01], the decline in the percentage of G(0)G(1) phase cells (0.69 +/- 0.24 in Met(-)Hcy(+) vs 0.80 +/- 0.18 in Met(+)Hcy(-), P<0.01) and the increase of S cells (0.24 +/- 0.20 in Met(-)Hcy(+) vs 0.17 +/- 0.16 in Met(+)Hcy(-), P<0.01); however, gastric mucosal cells grew normally. If Met(-)Hcy(+) medium was used in combination with chemotherapeutic drugs, the number of surviving gastric cancer...Continue Reading

References

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Jan 31, 2002·World Journal of Gastroenterology : WJG·Lin CaiZuo-Feng Zhang

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Citations

Dec 12, 2003·World Journal of Gastroenterology : WJG·Yin-Cheng HeYa-Kui Zhou
Mar 25, 2004·World Journal of Gastroenterology : WJG·Jie YanYa-Fei Mao
Jan 13, 2004·World Journal of Gastroenterology : WJG·Jia-Ren LiuYou-Qiang Song
Feb 5, 2004·World Journal of Gastroenterology : WJG·Jia-Ren LiuYou-Qiang Song
Aug 27, 2010·American Journal of Physiology. Cell Physiology·Arivudainambi RamalingamJames M Mullin
Sep 6, 2012·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Luigina GraziosiStefano Fiorucci
Jun 6, 2018·Frontiers in Oncology·Daniele Lettieri-Barbato, Katia Aquilano
Nov 20, 2002·World Journal of Gastroenterology : WJG·Jian-Kun HuYou-Ping Li
Jun 5, 2002·World Journal of Gastroenterology : WJG·Jun HouDeng-Gui Wen
Jul 11, 2003·World Journal of Gastroenterology : WJG·Ya-Fei MaoShu-Ping Li
Oct 17, 2003·World Journal of Gastroenterology : WJG·Shan GaoHe-Sheng Luo

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