Methionine oxidation induces amyloid fibril formation by full-length apolipoprotein A-I.

Proceedings of the National Academy of Sciences of the United States of America
Yuan Qi WongMichael D W Griffin

Abstract

Apolipoprotein A-I (apoA-I) is the major protein component of HDL, where it plays an important role in cholesterol transport. The deposition of apoA-I derived amyloid is associated with various hereditary systemic amyloidoses and atherosclerosis; however, very little is known about the mechanism of apoA-I amyloid formation. Methionine residues in apoA-I are oxidized via several mechanisms in vivo to form methionine sulfoxide (MetO), and significant levels of methionine oxidized apoA-I (MetO-apoA-I) are present in normal human serum. We investigated the effect of methionine oxidation on the structure, stability, and aggregation of full-length, lipid-free apoA-I. Circular dichrosim spectroscopy showed that oxidation of all three methionine residues in apoA-I caused partial unfolding of the protein and decreased its thermal stability, reducing the melting temperature (T(m)) from 58.7 degrees C for native apoA-I to 48.2 degrees C for MetO-apoA-I. Analytical ultracentrifugation revealed that methionine oxidation inhibited the native self association of apoA-I to form dimers and tetramers. Incubation of MetO-apoA-I for extended periods resulted in aggregation of the protein, and these aggregates bound Thioflavin T and Congo Red. Insp...Continue Reading

References

Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·A K SoutarJ J Hsuan
Apr 2, 1996·Proceedings of the National Academy of Sciences of the United States of America·O Gursky, D Atkinson
Jan 1, 1997·Advances in Protein Chemistry·J W KellyS A Peterson
Nov 14, 1997·Journal of Molecular Biology·M SundeC C Blake
Jan 14, 1999·The New England Journal of Medicine·R Ross
Jul 13, 2000·Biochemistry·D M HattersG J Howlett
Aug 18, 2000·Free Radical Biology & Medicine·G M Chisolm, D Steinberg
Feb 17, 2001·The Journal of Pathology·G I MucchianoP Westermark
Mar 27, 2001·Protein Science : a Publication of the Protein Society·P MangioneV Bellotti
Jun 14, 2002·FEBS Letters·Vladimir N UverskyAnthony L Fink
Aug 29, 2002·The Journal of Biological Chemistry·Liming HouMichael G Zagorski
Sep 20, 2002·The Journal of Biological Chemistry·Kathryn J MooreMason W Freeman
Nov 8, 2002·The Journal of Biological Chemistry·Alessia AndreolaMargaret Sunde
Feb 11, 2003·Journal of Lipid Research·Greg PankhurstRoland Stocker
Nov 1, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Kerry-Anne Rye, Philip J Barter
Dec 16, 2003·Current Opinion in Rheumatology·Joel N Buxbaum
Dec 31, 2003·The Journal of Biological Chemistry·Lea A MedeirosKathryn J Moore
Oct 16, 2004·Circulation Research·Philip J BarterAlan M Fogelman
Feb 1, 2005·Biochimica Et Biophysica Acta·Ute Panzenböck, Roland Stocker
Sep 27, 2005·Trends in Cardiovascular Medicine·Stephen J NichollsStanley L Hazen
Oct 14, 2005·Journal of the American Society of Nephrology : JASN·Gina GregoriniFrancesco Scolari
Feb 3, 2006·Proceedings of the National Academy of Sciences of the United States of America·A Abdul AjeesH M Krishna Murthy
Feb 18, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Christoph RöckenThomas Bürger
Sep 9, 2006·Current Opinion in Lipidology·Geoffrey J Howlett, Kathryn J Moore
Nov 17, 2006·Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis·Laura ObiciVittorio Bellotti
Nov 23, 2006·The FEBS Journal·Simin D MalekniaJoel N Buxbaum
Jan 19, 2008·Journal of Lipid Research·Jonathan W C BrockJohn W Baynes
Aug 23, 2008·Proceedings of the National Academy of Sciences of the United States of America·Baohai ShaoJay W Heinecke
Sep 5, 2008·European Biophysics Journal : EBJ·Andrew HungIrene Yarovsky

❮ Previous
Next ❯

Citations

Mar 15, 2011·Protein & Cell·Chai Lean TeohGeoffrey J Howlett
Mar 17, 2012·Journal of Proteome Research·Chunchao ZhangPhilip C Andrews
Aug 13, 2011·The Journal of Biological Chemistry·Shobini JayaramanGiorgio Cavigiolio
Jun 5, 2012·The Journal of Biological Chemistry·Jung Chae LimRodney L Levine
Jan 5, 2013·The Journal of Biological Chemistry·Xiaodi DengThomas B Thompson
Apr 14, 2015·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Sung-Jin ParkYoung-Tae Chang
Oct 31, 2012·Molecular Aspects of Medicine·Niki ChondrogianniEfstathios S Gonos
Jan 12, 2011·Molecular Microbiology·Michela CarellaMatthias Görlach
Nov 2, 2013·Mass Spectrometry Reviews·Bart Ghesquière, Kris Gevaert
Feb 12, 2011·The Biochemical Journal·Simon V Avery
Aug 16, 2016·Journal of Molecular Recognition : JMR·F TaghaviA A Moosavi-Movahedi
Oct 30, 2016·FEBS Letters·Angela ArcielloDaria Maria Monti
Nov 26, 2015·Scientific Reports·Juan C AledoFrancisco J Veredas
May 9, 2014·Biochemistry·Michel de MessieresJennifer C Lee
Dec 8, 2017·Cell Stress & Chaperones·Hui-Yong LianPeng Cai
Jun 2, 2018·The Journal of Biological Chemistry·David TownsendDavid A Middleton
Jul 6, 2011·Free Radical Research·Eric O Uthus, Matthew J Picklo
Aug 3, 2014·The Journal of Biological Chemistry·Andre L SamsonRobert L Medcalf
Jul 16, 2015·The Journal of Biological Chemistry·Chiharu MizuguchiHiroyuki Saito
Feb 7, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Andrzej WitkowskiGiorgio Cavigiolio
Apr 21, 2020·Biochemical Society Transactions·Loes van Dam, Tobias B Dansen
Feb 28, 2020·Arthritis & Rheumatology·Minzhi YuAnna Schwendeman
Aug 15, 2012·The Journal of Biological Chemistry·Yasushi MitomiYoshiaki Furukawa
Mar 12, 2015·The Journal of Biological Chemistry·Gary K L ChanGiorgio Cavigiolio
Jan 13, 2019·The Journal of Biological Chemistry·Andrzej WitkowskiGiorgio Cavigiolio

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved