Methionine sulfoxide reductase A (MsrA) deficient Mycoplasma genitalium shows decreased interactions with host cells.

PloS One
Kishore DasSubramanian Dhandayuthapani

Abstract

Mycoplasma genitalium is an important sexually transmitted pathogen that affects both men and women. In genital-mucosal tissues, it initiates colonization of epithelial cells by attaching itself to host cells via several identified bacterial ligands and host cell surface receptors. We have previously shown that a mutant form of M. genitalium lacking methionine sulfoxide reductase A (MsrA), an antioxidant enzyme which converts oxidized methionine (Met(O)) into methionine (Met), shows decreased viability in infected animals. To gain more insights into the mechanisms by which MsrA controls M. genitalium virulence, we compared the wild-type M. genitalium strain (G37) with an msrA mutant (MS5) strain for their ability to interact with target cervical epithelial cell lines (HeLa and C33A) and THP-1 monocytic cells. Infection of epithelial cell lines with both strains revealed that MS5 was less cytotoxic to HeLa and C33A cell lines than the G37 strain. Also, the MS5 strain was more susceptible to phagocytosis by THP-1 cells than wild type strain (G37). Further, MS5 was less able to induce aggregation and differentiation in THP-1 cells than the wild type strain, as determined by carboxyfluorescein diacetate succinimidyl ester (CFSE) la...Continue Reading

References

Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J S BeckmanB A Freeman
Oct 20, 1995·Science·C M FraserJ C Venter
Aug 1, 1995·Microbial Pathogenesis·J B BasemanC A Thomas
Jul 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·T M WizemannH R Masure
Oct 1, 1996·American Journal of Respiratory and Critical Care Medicine·J B BasemanS F Dallo
May 29, 1998·Infection and Immunity·P CaoT L Cover
Feb 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·M E HassouniF Barras
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·S DhandayuthapaniJ B Baseman
Jun 22, 1999·Infection and Immunity·L ReyesJ K Davis
Sep 2, 1999·Biochemical and Biophysical Research Communications·H TatsuzawaM Nakano
Jul 7, 2000·Clinical and Diagnostic Laboratory Immunology·E M AbdoJ Frey
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·C Nathan, M U Shiloh
Oct 14, 2000·Microbial Pathogenesis·S F Dallo, J B Baseman
Sep 7, 2001·Journal of Bacteriology·S DhandayuthapaniJ B Baseman
Oct 26, 2001·The Journal of Biological Chemistry·R GrimaudF Barras
Feb 14, 2002·Archives of Biochemistry and Biophysics·Herbert WeissbachNathan Brot
Feb 14, 2002·Archives of Biochemistry and Biophysics·B M BabiorW Nauseef
Mar 13, 2002·Lancet·Craig R CohenPatricia A Totten
Apr 9, 2002·Nature Structural Biology·W Todd LowtherBrian W Matthews
Jul 4, 2002·Proceedings of the National Academy of Sciences of the United States of America·Eric P SkaarH Steven Seifert
Jul 30, 2002·The Journal of Biological Chemistry·R Abhilash KumarVadim N Gladyshev
Jan 21, 2004·Molecular Microbiology·Christophe BeloinJean-Marc Ghigo
Feb 11, 2004·International Journal of STD & AIDS·D Taylor-RobinsonP E Hay
Dec 22, 2004·The Plant Journal : for Cell and Molecular Biology·Pascal ReyMélanie Broin
Feb 1, 2005·Biochimica Et Biophysica Acta·Herbert WeissbachNathan Brot
Feb 1, 2005·Biochimica Et Biophysica Acta·Benjamin EzratyFrédéric Barras
Feb 1, 2005·Biochimica Et Biophysica Acta·Sandrine Boschi-MullerGuy Branlant
Jan 26, 2006·Journal of Leukocyte Biology·Felix I L ClanchyJohn A Hamilton

❮ Previous
Next ❯

Citations

Feb 26, 2013·BMC Microbiology·Mario A MartinezSubramanian Dhandayuthapani
May 11, 2015·FEBS Letters·Isabelle S ArtsJean-François Collet
Mar 26, 2014·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Kishore DasSubramanian Dhandayuthapani
Apr 20, 2017·Nature Reviews. Microbiology·Benjamin EzratyJean-François Collet
Oct 29, 2021·Frontiers in Immunology·Enrique I RamosSubramanian Dhandayuthapani

❮ Previous
Next ❯

Methods Mentioned

BETA
Assay
PMA
ELISA
Protein Assay

Software Mentioned

- J
NIH Image J
Image
Image J
NIH

Related Concepts

Related Feeds

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Babesiosis

Babesiosis is caused by parasites of the genus babesia, which are transmitted in nature by the bite of an infected tick. Discover the latest research on babesiosis here.

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.