Method and Apparatus for the Automated Delivery of Continuous Neural Stem Cell Trails Into the Spinal Cord of Small and Large Animals

Neurosurgery
Artem B KutikovJames D Guest

Abstract

Immature neurons can extend processes after transplantation in adult animals. Neuronal relays can form between injected neural stem cells (NSCs) and surviving neurons, possibly improving recovery after spinal cord injury (SCI). Cell delivery methods of single or multiple bolus injections of concentrated cell suspensions thus far tested in preclinical and clinical experiments are suboptimal for new tract formation. Nonuniform injectate dispersal is often seen due to gravitational cell settling and clumping. Multiple injections have additive risks of hemorrhage, parenchymal damage, and cellular reflux and require additional surgical exposure. The deposition of multiply delivered cells boluses may be uneven and discontinuous. To develop an injection apparatus and methodology to deliver continuous cellular trails bridging spinal cord lesions. We improved the uniformity of cellular trails by formulating NSCs in hyaluronic acid. The TrailmakerTM stereotaxic injection device was automatized to extend a shape memory needle from a single-entry point in the spinal cord longitudinal axis to "pioneer" a new trail space and then retract while depositing an hyaluronic acid-NSC suspension. We conducted testing in a collagen spinal models, and...Continue Reading

References

Jan 14, 1975·Biochemistry·R U MargolisC Preti
Aug 1, 1989·Archives of Ophthalmology·G C Brown, W E Benson
Jan 24, 1998·Experimental Neurology·M S BeattieW Young
Apr 17, 2001·Clinical Orthopaedics and Related Research·K D BrandtP T Lavin
Feb 9, 2002·Science·Martin E Schwab
Nov 21, 2002·Progress in Brain Research·Barbara S BregmanFaheem Sandhu
Feb 18, 2004·Nature Neuroscience·Florence M BareyreMartin E Schwab
Mar 6, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Lucas CamposJohn H Martin
Jul 22, 2006·Nature Reviews. Neuroscience·Glenn Yiu, Zhigang He
Jan 16, 2007·Current Opinion in Neurobiology·Sarah A Busch, Jerry Silver
Oct 20, 2007·Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society·J A EnghC N Riviere
Oct 31, 2008·Current Protocols in Neuroscience·Simon W Moore, Timothy E Kennedy
Jun 16, 2009·Journal of Visualized Experiments : JoVE·Gerlinde A Metz, Ian Q Whishaw
May 22, 2010·Journal of Neurotrauma·Maayken E L van den BergIgnacio Mahillo-Fernandez
Jan 25, 2011·American Journal of Infection Control·Gwo-Hwa WanChin-Sheng Tang
May 31, 2011·Frontiers in Bioscience (Scholar Edition)·Marnie Preston, Larry S Sherman
Jul 15, 2011·Journal of Neural Engineering·Zin Z KhaingChristine E Schmidt
Oct 21, 2011·Tissue Engineering. Part a·Brian A AguadoSarah C Heilshorn
Mar 20, 2013·Biomatter·Pouria Moshayedi, S Thomas Carmichael

❮ Previous
Next ❯

Citations

Mar 13, 2021·World Journal of Stem Cells·Pavan S UpadhyayulaJoseph D Ciacci

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

Related Papers

Journal of Environmental Management
Catherine Marina Pickering, Patrick Norman
The New England Journal of Medicine
R Buxbaum, R O'Connor
Scientific American
Christina Reed
© 2022 Meta ULC. All rights reserved