Method comparison for enhanced recovery, isolation and qualitative detection of C. jejuni and C. coli from wastewater effluent samples

International Journal of Environmental Research and Public Health
M Ugarte-RuizL Domínguez

Abstract

Seeking a sensitive protocol, culture-dependent methods were compared to detect thermophilic Campylobacter species in untreated urban effluents. We evaluated various combinations of selective media, with and without an enrichment steps, as well as an extra filtration step. Culture-independent real-time quantitative PCR was also included and all detected isolates underwent antimicrobial susceptibility testing. All tested water samples contained Campylobacter DNA, but only 64% were positive after culture. Although enrichment using Preston broth resulted in better recovery of potentially stressed Campylobacter than Bolton or Campyfood broth (CFB), there was no significant increase in efficiency compared to direct plating. The type of selective agar media used, on the other hand, had a significant effect, with CASA plates performing better than mCCDA or CFA ones. Inclusion of an enrichment step increased the ratio of C. coli vs. C. jejuni being isolated. Resistances against all antimicrobials tested were observed in C. coli, but fewer instances of resistance were found in C. jejuni isolates. Whether this difference was the result of selection during the enrichment step could not be determined. The presence of Campylobacter in urban...Continue Reading

References

Oct 1, 1991·Journal of General Microbiology·D M JonesA Curry
Jul 1, 1991·Canadian Journal of Microbiology·L K Korhonen, P J Martikainen
Feb 1, 1991·Journal of Clinical Microbiology·J A KiehlbauchI K Wachsmuth
Sep 1, 1986·Applied and Environmental Microbiology·D M Rollins, R R Colwell
Dec 19, 2000·Journal of Applied Microbiology·C L BaylisR P Betts
Apr 3, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·B M Allos
May 26, 2001·Applied and Environmental Microbiology·D G NewellG Mead
Jun 26, 2001·Symposium Series·J A Frost
Feb 7, 2003·Applied and Environmental Microbiology·Yolanda MorenoJavier Hernández
Oct 22, 2004·Emerging Infectious Diseases·Daniela Schönberg-NorioHilpi Rautelin
May 24, 2006·Microbes and Infection·John E MooreOla Tolba
Sep 19, 2006·International Journal of Food Microbiology·Insook SonMark A Harrison
Dec 19, 2006·Applied and Environmental Microbiology·Ethell VereenErin K Lipp
Dec 22, 2006·Environmental Science & Technology·Hussein H AbulreeshRaymond Goulder
Jun 26, 2007·Applied and Environmental Microbiology·Andreas NockerAnne K Camper
Jun 7, 2008·Current Opinion in Biotechnology·Fernando BaqueroRafael Cantón
Sep 24, 2008·Poultry Science·E D van AsseltH J van der Fels-Klerx
Sep 9, 2009·Journal of Applied Microbiology·S BörjessonP E Lindgren
Feb 26, 2010·International Journal of Environmental Health Research·Andrea Rechenburg, Thomas Kistemann
Dec 1, 1998·Journal of Applied Microbiology·C ThomasM Mabey
Feb 4, 2011·Epidemiologic Perspectives & Innovations : EP+I·Joseph H Abramson
Sep 20, 2011·Epidemiology and Infection·L K WilliamsT J Humphrey
Oct 26, 2011·Nature Reviews. Gastroenterology & Hepatology·Si Ming Man
May 15, 2012·International Journal of Antimicrobial Agents·Martine DenisIsabelle Kempf
Feb 9, 2013·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·David Pérez-BotoM Aurora Echeita
Feb 15, 2013·Virulence·Nicole M Iovine
Jul 3, 2013·Journal of Microbiological Methods·Tarja Pitkänen
Jul 3, 2013·Journal of Microbiological Methods·Ewa PacholewiczBirgitta Duim
Nov 13, 2013·International Journal of Environmental Research and Public Health·Harriet WhileyRichard Bentham
Nov 30, 2013·International Journal of Environmental Research and Public Health·Sharon V R EppsDavid J Nisbet

❮ Previous
Next ❯

Citations

Nov 26, 2015·The Journal of Antimicrobial Chemotherapy·Diego Florez-CuadradoM Concepción Porrero
Apr 24, 2016·Environmental Science and Pollution Research International·Si BonettaE Carraro
Aug 7, 2019·Environmental Microbiology·Evangelos MourkasSamuel K Sheppard
Dec 20, 2019·Canadian Journal of Microbiology·Maxim SheludchenkoHelen Stratton
Jun 27, 2019·International Journal of Environmental Research and Public Health·Martina O ChukwuJohn Barr Dewar
Jul 3, 2021·International Journal of Environmental Research and Public Health·Nicol StrakovaRenata Karpiskova

❮ Previous
Next ❯

Datasets Mentioned

BETA
LN811437

Methods Mentioned

BETA
PCR
PMA

Software Mentioned

R
WinPepi
MALDI [UNK]
WinEpi
EzTaxon
ClustalW
R Development Core Team

Related Concepts

Related Feeds

Campylobacteriosis (ASM)

Campylobacteriosis is caused by the bacteria Campylobacter jejuni and is a common cause of gastroenteritis in humans. Discover the latest research on Campylobacteriosis here.