Method development and validation for isoflavones in soy germ pharmaceutical capsules using micellar electrokinetic chromatography

Journal of Pharmaceutical and Biomedical Analysis
Gustavo Amadeu MickeMarina Franco Maggi Tavares

Abstract

The separation of six soy isoflavones (Glycitein, Daidzein, Genistein, Daidzin, Glycitin and Genistin) was approached by a 3(2) factorial design studying MEKC electrolyte components at the following levels: methanol (MeOH; 0-10%) and sodium dodecylsulfate (SDS; 20-70 mmol L(-1)); sodium tetraborate buffer (STB) concentration was kept constant at 10 mmol L(-1). Nine experiments were performed and the apparent mobility of each isoflavone was computed as a function of the electrolyte composition. A novel response function (RF) was formulated based on the production of the mobility differences, mobility of the first and last eluting peaks and the electrolyte conductance. The inspection of the response surface indicated an optimum electrolyte composition as 10 mmol L(-1) STB (pH 9.3) containing 40 mmol L(-1) SDS and 1% MeOH promoting baseline separation of all isoflavones in less than 7.5 min. The proposed method was applied to the determination of total isoflavones in soy germ capsules from four different pharmaceutical laboratories. A 2h extraction procedure with 80% (v/v) MeOH under vortexing at room temperature was employed. Peak assignment of unknown isoflavones in certain samples was assisted by hydrolysis procedures, migratio...Continue Reading

References

Oct 3, 2003·Journal of Chromatography. a·Mauricio A RostagnoCarmelo G Barroso
Jun 3, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Borivoj KlejdusVlastimil Kubán
Jul 6, 2004·Journal of Chromatography. a·Sandra ApersArnold Vlietinck

❮ Previous
Next ❯

Citations

Aug 20, 2010·Journal of Pharmaceutical and Biomedical Analysis·Elena Hurtado-FernándezAlberto Fernández-Gutiérrez
Apr 12, 2016·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Catharina Nucci-MartinsAdair R S Santos
Dec 3, 2014·Journal of Pharmaceutical and Biomedical Analysis·Sylwia MagieraAnna Lautenszleger
Sep 8, 2012·Food Chemistry·Svitlana ShinkarukCatherine Bennetau-Pelissero
Jun 10, 2016·Journal of Chromatographic Science·Wen XiaoFeng-Qing Yang
Nov 28, 2007·Electrophoresis·Virginia García-Cañas, Alejandro Cifuentes
May 8, 2008·Journal of Separation Science·Maria Addolorata SaracinoMaria Augusta Raggi
Apr 21, 2016·The Cochrane Database of Systematic Reviews·Devashana GuptaVicki Nisenblat
Jul 24, 2008·Journal of Agricultural and Food Chemistry·Svitlana ShinkarukCatherine Bennetau-Pelissero

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.