Method development for the quantitation of ABT-578 in rabbit artery tissue by 96-well liquid-liquid extraction and liquid chromatography/tandem mass spectrometric detection

Rapid Communications in Mass Spectrometry : RCM
Qin C JiTawakol El-Shourbagy

Abstract

Quantitative determination of drug concentrations in tissue samples can provide critical information for drug metabolism, kinetics, and toxicity evaluations. For analysis of tissue samples using liquid chromatography/tandem mass spectrometric (LC/MS/MS) detection, homogenization is a critical step in achieving good assay performance. Assay performance can be closely evaluated by spiking the drug directly into tissue samples prior to homogenization. It is especially important to include this assay evaluation for the analysis of artery tissue samples because artery tissue is very elastic, making it quite a challenge to develop an effective procedure for homogenization. An LC/MS/MS assay in 96-well format using liquid-liquid extraction was developed for analyzing ABT-578 in rabbit artery samples. Tissue quality control samples were prepared by spiking ABT-578 stock solutions directly into the tissue before homogenization. The usage of the tissue control samples gives a thorough evaluation of the sample preparation process that includes both homogenization and sample extraction. A 20% blood in saline solution was used as a homogenization solution. Calibration standards were made by spiking ABT-578 into rabbit whole blood. Blood qua...Continue Reading

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Apr 29, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Qin C JiTawakol A El-Shourbagy

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Citations

Feb 13, 2007·Analytical and Bioanalytical Chemistry·Jun ZhangLewis Schwartz
Nov 24, 2012·Bioanalysis·Y-J XueNaidong Weng
Jul 8, 2009·Journal of Pharmaceutical and Biomedical Analysis·Quan ChenCasey Chun Zhou
Dec 22, 2004·The Journal of Physiology·Leigh Anderson

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