Method for assessment of vascular reactivity in bone: in vitro studies on resistance arteries isolated from porcine cancellous bone

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
Anette LundgaardE S Hansen

Abstract

Knowledge about vascular regulation in bone is central to the understanding of both normal and pathological bone physiology. This article describes a new method for direct assessment of the reactivity of bone blood vessels. Resistance arteries (diameter approximately 250 microns) were isolated from epiphyseal cancellous bone (porcine femoral condyle). Arterial segments (2 mm long) were mounted as ring preparations on a myograph, and isometric force development was measured continuously. Fifty-nine vessels from 31 pigs were investigated. The active force development was maximal at 0.9 x L100 in nine of 12 investigated arteries (L100 corresponds to the circumference the vessel would have if relaxed and exposed to a luminal pressure of 100 mm Hg [13.3 kPa]). In all subsequent experiments, the vessels were stretched to 0.9 x L100. Noradrenaline (2 x 10(-8) to 10(-5) M) induced a concentration-dependent vasoconstriction; mean maximal tension development was 3.69 N/m. This force development would enable the arteries to contract against a pressure of more than 22 kPa (165 mm Hg), indicating preserved function of the media smooth muscle. Response to acetylcholine (10(-7) to 10(-5) M) was observed in only two of 12 arteries. Bradykinin ...Continue Reading

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Citations

May 3, 2001·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·A LundgaardE S Hansen
Jun 12, 2001·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·V B HansenE S Hansen
Nov 3, 2009·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Wolf DrescherThomas Pufe
Apr 7, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Wolf DrescherMahmed Kadyrov
Mar 30, 2005·The Journal of Bone and Joint Surgery. British Volume·A C DischC Perka
Sep 2, 2008·The Journal of Bone and Joint Surgery. British Volume·N-F ZhangP Hernigou
Jul 3, 2004·Clinical Orthopaedics and Related Research·Wolf DrescherEbbe S Hansen
Nov 3, 2006·The Journal of Bone and Joint Surgery. American Volume·Wolf DrescherEbbe-Stender Hansen
Nov 3, 2006·The Journal of Bone and Joint Surgery. American Volume·Wolf DrescherThomas Pufe

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