Method for Biomonitoring DNA Adducts in Exfoliated Urinary Cells by Mass Spectrometry

Analytical Chemistry
Byeong Hwa YunRobert J Turesky

Abstract

Tobacco smoking contributes to about 50% of the bladder-cancer (BC) cases in the United States. Some aromatic amines in tobacco smoke are bladder carcinogens; however, other causal agents of BC are uncertain. Exfoliated urinary cells (EUCs) are a promising noninvasive biospecimen to screen for DNA adducts of chemicals that damage the bladder genome, although the analysis of DNA adducts in EUCs is technically challenging because of the low number of EUCs and limiting quantity of cellular DNA. Moreover, EUCs and their DNA adducts must remain viable during the time of collection and storage of urine to develop robust screening methods. We employed RT4 cells, a well-differentiated transitional epithelial bladder cell line, as a cell-model system in urine to investigate cell viability and the chemical stability of DNA adducts of two prototypical bladder carcinogens: 4-aminobiphenyl (4-ABP), an aromatic amine found in tobacco smoke, and aristolochic acid I (AA-I), a nitrophenanthrene found in Aristolochia herbaceous plants used for medicinal purposes worldwide. The cell viability of RT4 cells pretreated with 4-ABP or AA-I in urine exceeded 80%, and the major DNA adducts of 4-ABP and AA-I, quantified by liquid chromatography-mass spec...Continue Reading

References

Jan 1, 1978·Biophysical Journal·W D Corry, H J Meiselman
Jun 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·G TalaskaF F Kadlubar
Oct 1, 1989·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·D J DoolittleA W Hayes
May 14, 1996·Proceedings of the National Academy of Sciences of the United States of America·N RothmanG Talaska
Mar 18, 2000·The Science of the Total Environment·G GrimmerJ Jacob
Sep 28, 2002·Mutation Research·Mimi C YuRonald K Ross
Oct 21, 2003·Chemical Research in Toxicology·Christopher R Jones, Gabriele Sabbioni
Nov 17, 2005·Carcinogenesis·R A CrallanJ Southgate
Sep 22, 2006·International Journal of Cancer. Journal International Du Cancer·Miki NakajimaTsuyoshi Yokoi
Jul 11, 2007·Proceedings of the National Academy of Sciences of the United States of America·Arthur P GrollmanBojan Jelaković
Jun 24, 2008·Journal of Toxicology and Environmental Health. Part a·Rouslana BelikKlaus Golka
Sep 12, 2009·Critical Reviews in Toxicology·Annie M JarabekErrol Zeiger
Mar 25, 2011·American Journal of Physiology. Renal Physiology·Thomas A Rosenquist
Jun 25, 2013·Cell Biochemistry and Biophysics·Haijiao LiuYu Sun
May 28, 2015·Genome Medicine·Song Ling PoonSteven G Rozen
Sep 15, 2015·Toxicology Research·Byeong Hwa YunRobert J Turesky
Oct 11, 2016·Experimental Cell Research·Galina ReshetnikovaVictor Romanov
Dec 8, 2016·Journal of Toxicology and Environmental Health. Part a·Sabine PlöttnerThomas Brüning

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Citations

May 17, 2019·High-throughput·Jingshu Guo, Robert J Turesky
Jul 24, 2020·Current Opinion in Urology·Katharina RebhanThomas Rosenquist

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