Method for determination of methadone in exhaled breath collected from subjects undergoing methadone maintenance treatment

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Olof BeckGöran Palmskog

Abstract

At present drugs of abuse testing using exhaled breath as specimen is only possible for alcohol. However, we recently discovered that using modern liquid chromatography-mass spectrometry technique amphetamine and methamphetamine is detectable in exhaled breath following intake in drug addicts. We therefore undertook to develop a method for determination of methadone in exhaled breath from patients undergoing methadone maintenance treatment. Exhaled breath was collected from 13 patients after intake of the daily methadone dose. The compounds were trapped by filtering the air through a C18 modified silica surface. After elution of any trapped methadone the extract was analysed by a combined liquid chromatography-tandem mass spectrometry method. Recovery of trapped methadone from the filter surface was 96%, no significant matrix effect was observed, and the quantification using methadone-d3 as an internal standard was accurate (<10% bias) and precise (coefficient of variation 1.6-2.0%). Methadone was indisputably identified by means of the mass spectrometry technique in exhaled breath samples from all 13 patients. Identification was based on monitoring two product ions in selected reaction monitoring mode with correct relative rat...Continue Reading

References

Oct 2, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Barbora Maralikova, Wolfgang Weinmann
Jun 26, 2007·Journal of Substance Abuse Treatment·William B JaffeeRoger D Weiss
Jan 8, 2009·Current Pain and Headache Reports·Jodie A Trafton, Abhinav Ramani

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Citations

Jun 16, 2012·Analytical and Bioanalytical Chemistry·Maria Addolorata SaracinoMaria Augusta Raggi
Jan 7, 2015·Scandinavian Journal of Clinical and Laboratory Investigation·Sten CarlssonOlof Beck
Aug 30, 2011·Journal of Pharmaceutical and Biomedical Analysis·Olof BeckJohan Franck
Jan 21, 2014·Science & Justice : Journal of the Forensic Science Society·Olof Beck
Oct 15, 2014·Journal of Substance Abuse Treatment·Charlotte SkoglundOlof Beck
Feb 18, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Niclas StephansonOlof Beck
Oct 10, 2012·Journal of Analytical Toxicology·Olof BeckJohan Franck
Feb 15, 2018·Journal of Breath Research·Sabina SeferajOlof Beck
Mar 27, 2018·Drug Testing and Analysis·Michel ArvidssonOlof Beck
Dec 9, 2017·Journal of Breath Research·Göran LjungkvistAnna-Carin Olin
Feb 22, 2017·Journal of Breath Research·Phillip TrefzWolfram Miekisch
Jan 13, 2019·Respiratory Research·B BakeA-C Olin
Sep 4, 2020·Rapid Communications in Mass Spectrometry : RCM·Ann-Marie GarzinskyMario Thevis
Oct 9, 2019·Chemical Reviews·Tobias BrudererRenato Zenobi

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