PMID: 7029130Oct 1, 1981Paper

Method for direct induction of serotonin fluorescence in a carcinoid tumor after embedding in hydroxyethyl methacrylate

Laboratory Investigation; a Journal of Technical Methods and Pathology
H LyonM Møller

Abstract

Tissue slices from a carcinoid tumor were fixed in formalin and after dehydration embedded in hydroxyethyl methacrylate. Sections, 5 micrometers in thickness, were cut with a D-knife mounted on a serial microtome. Some sections were stained with either hematoxylin and eosin or by azo-coupling with fast garnet GBC, whereas others were reacted for catecholamines and indolamines either by exposing them to formaldehyde vapor for 1 hour at 80 degrees C. or by dipping them into a sucrose-phosphate buffer-glyoxylic acid solution followed by heating at 80 degrees C. for 15 minutes. Fluorescence intensities and the spectra of the fluorophores were recorded on 5-micrometers thick formaldehyde-reacted sections by microspectrofluorometry. With both the formaldehyde vapor method and the glyoxylic acid solution method, an intense yellow fluorescence was obtained from the carcinoid tumor cells, as well as from normal enterochromaffin cells. The excitation and emission maxima obtained by microspectrofluorometry from these cells indicated the presence of a fluorophore originating from serotonin.

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.