Method for extraction of high-quantity and -quality cell-free DNA from amniotic fluid.

Methods in Molecular Biology
Olav LapaireDiana W Bianchi

Abstract

Circulating cell-free fetal deoxyribonucleic acids (cffDNAs) are promising biomarkers with various potential clinical applications. Second and third trimester amniotic fluid (AF) is a rich source of cffDNAs. Further improvements to the original protocol for the extraction of cffDNAs from AF supernatant resulted in statistically significant higher yields of high-quality cffDNAs, allowing for a substantial majority of samples to be analyzed with subsequent molecular methods (e.g., comparative genomic hybridization microarrays) to further assess for genetic abnormalities. Several advantages have been realized with the optimized protocol. In addition to an improved yield from a greater proportion of samples compared with the original protocol, the current method, using large silico-membranes, allows for the extraction of cffDNAs from up to 10 samples in <3 h. The replacement of the original lysis buffer eliminates the need for a heating bath during the lysis step, and fewer overall steps are involved in the protocol (e.g., to reduce potential contamination). The improvements in the yield with the current protocol make it possible to augment current standard of care through the analysis of this previously unappreciated source of gen...Continue Reading

Citations

Oct 7, 2010·Human Reproduction Update·L Hui, D W Bianchi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Birth Defects

Birth defects encompass structural and functional alterations that occur during embryonic or fetal development and are present since birth. The cause may be genetic, environmental or unknown and can result in physical and/or mental impairment. Here is the latest research on birth defects.

Related Papers

Progress in Neurobiology
Rory JohnsonNoel J Buckley
Proceedings of the National Academy of Sciences of the United States of America
B J TurnerD S Taylor
Langmuir : the ACS Journal of Surfaces and Colloids
Annette GranéliEric C Greene
© 2022 Meta ULC. All rights reserved