Method for functional study of mitochondria in rat hypothalamus

Journal of Neuroscience Methods
Alexandre BenaniLuc Pénicaud

Abstract

Different roles of mitochondria in brain function according to brain area are now clearly emerging. Unfortunately, no technique is yet described to investigate mitochondria function in specific brain area. In this article, we provide a complete description of a procedure to analyze the mitochondrial function in rat brain biopsies. Our two-step method consists in a saponin permeabilization of fresh brain tissues in combination with high-resolution respirometry to acquire the integrated respiratory rate of the biopsy. In the first part, we carefully checked the mitochondria integrity after permeabilization, defined experimental conditions to determine the respiratory control ratio (RCR), and tested the reproducibility of this technique. In the second part, we applied our method to test its sensitivity. As a result, this method was sensitive enough to reveal region specificity of mitochondrial respiration within the brain. Moreover, we detected physiopathological modulation of the mitochondrial function in the hypothalamus. Thus this new technique that takes all cell types into account, and does not discard or select any mitochondria sub-population is very suitable to analyze the integrated mitochondrial respiration of brain biops...Continue Reading

References

Jul 1, 1977·Archives of Biochemistry and Biophysics·S S KatyareA Sreenivasan
May 1, 1966·Journal of Biochemistry·K OzawaC Araki
Jul 1, 1994·Clinica Chimica Acta; International Journal of Clinical Chemistry·P RustinA Munnich
Nov 5, 1997·Analytical Biochemistry·H N RasmussenU F Rasmussen
Feb 15, 2000·Annals of the New York Academy of Sciences·H S WaagepetersenA Schousboe
Jun 17, 2000·Trends in Neurosciences·M F Beal
Jul 12, 2003·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·R Birkedal, H Gesser
Jun 16, 2004·Journal of Neuroscience Methods·Dzhamilja SafiulinaEnn Seppet
Aug 11, 2004·Molecular Aspects of Medicine·Michael R Duchen
Nov 10, 2004·Molecular and Cellular Biochemistry·Michael J Goldenthal, José Marín-García
Feb 8, 2005·Neuroscience Letters·Bruno Lobão-SoaresRoger Walz
Apr 12, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Alessandro RivaCharles Hoppel
Oct 15, 2005·Nature Reviews. Neuroscience·Zane B AndrewsTamas L Horvath
Nov 1, 2005·Free Radical Biology & Medicine·María A MoroIgnacio Lizasoain
Apr 7, 2006·Biochimica Et Biophysica Acta·Francis E SluseClaudine M Sluse-Goffart
Jun 30, 2006·American Journal of Physiology. Cell Physiology·G BenardR Rossignol
Jul 25, 2006·Current Biology : CB·Heidi M McBrideSylwia Wasiak
Sep 5, 2006·Medicinal Chemistry·P I MoreiraM S Santos
Oct 7, 2006·Neurochemical Research·Mark P Mattson
Apr 25, 2007·Journal of Neuroscience Research·David G NichollsNagendra Yadava
Apr 9, 2008·Physiological Reviews·Richard C Scarpulla
May 27, 2008·Biochimica Et Biophysica Acta·Grégory Mathy, Francis E Sluse
Jun 10, 2008·Nature Protocols·Andrey V KuznetsovWolfram S Kunz

❮ Previous
Next ❯

Citations

Jan 11, 2012·Antioxidants & Redox Signaling·Lionel CarneiroCorinne Leloup
Jan 11, 2013·Physiological and Biochemical Zoology : PBZ·Kirsten Gallagher, James F Staples
Jul 8, 2009·Diabetes·Anne-Laure ColombaniCorinne Leloup
Jun 1, 2016·Frontiers in Physiology·Christopher P KovachDebra A Fadool
Jan 13, 2017·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Mallory A BallingerMatthew T Andrews
Jun 16, 2011·Archives of Physiology and Biochemistry·Pablo M Garcia-Roves

❮ Previous
Next ❯

Related Concepts

Related Feeds

ATP Synthases

ATP synthases are enzymes located in the inner mitochondrial membrane that catalyze the synthesis of ATP during cellular respiration. Discover the latest research on ATP synthases here.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.