Method for inducing experimental pneumococcal meningitis in outbred mice

BMC Microbiology
Damiana ChiavoliniSusanna Ricci

Abstract

Streptococcus pneumoniae is the leading cause of bacterial meningitis. Pneumococcal meningitis is associated with the highest mortality among bacterial meningitis and it may also lead to neurological sequelae despite the use of antibiotic therapy. Experimental animal models of pneumococcal meningitis are important to study the pathogenesis of meningitis, the host immune response induced after infection, and the efficacy of novel drugs and vaccines. In the present work, we describe in detail a simple, reproducible and efficient method to induce pneumococcal meningitis in outbred mice by using the intracranial subarachnoidal route of infection. Bacteria were injected into the subarachnoid space through a soft point located 3.5 mm rostral from the bregma. The model was tested with several doses of pneumococci of three capsular serotypes (2, 3 and 4), and mice survival was recorded. Lethal doses killing 50 % of animals infected with type 2, 3 and 4 S. pneumoniae were 3.2 x 10, 2.9 x 10 and 1.9 x 10(2) colony forming units, respectively. Characterisation of the disease caused by the type 4 strain showed that in moribund mice systemic dissemination of pneumococci to blood and spleen occurred. Histological analysis of the brain of ani...Continue Reading

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Citations

Oct 17, 2007·Acta Neuropathologica·Denis GrandgirardStephen L Leib
Oct 16, 2008·Clinical Microbiology Reviews·Damiana ChiavoliniSusanna Ricci
Sep 26, 2013·BMC Infectious Diseases·Susanna RicciGianni Pozzi
Dec 10, 2015·Frontiers in Microbiology·Elena PettiniGianni Pozzi
Aug 24, 2006·Molecular Microbiology·Marco R OggioniGianni Pozzi
Mar 24, 2016·Acta Neuropathologica Communications·Joo-Yeon Engelen-LeeDiederik van de Beek
Mar 21, 2007·Neuroscience·T DavidsenT Tønjum
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Jan 12, 2021·JCI Insight·Babak BabanKrishnan M Dhandapani
Jul 4, 2019·ACS Infectious Diseases·Colin Kietzman, Elaine Tuomanen

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Methods Mentioned

BETA
light microscopy

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