PMID: 6979160Apr 1, 1982Paper

Method for isolation of gardnerella vaginalis (Haemophilus vaginalis). Characterization of isolates by gas chromatography

Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section B, Microbiology
P A CsángóG Jagars

Abstract

A method for the isolation of Gardnerella vaginalis (Haemophilus vaginalis) is presented. Bacteria isolated from 48-hour cultures grown on human blood agar were identified by means of beta-hemolysis, colony morphology, sensitivity to antimicrobial agents, oxydase and catalase reactions. Thirty-eight clinical isolates and one test strain were examined for fatty acid composition. Hexadecanoic (16:0), octadecenoic (18:1) and octadecanoic (18:0) were the major fatty acids. Also present, but in minor quantities, were myristic (14:0), hexadecenoic (16:1) and octadecadienoic (18:2) acids. Only insignificant differences between isolates could be detected. No hydroxy fatty acids commonly found in gram-negative bacteria were encountered. Gas chromatographic analysis of G. vaginalis revealed a characteristic and relatively simple pattern. The results support the use of the isolation method, which provides conditions highly selective for G. vaginalis.

References

Apr 1, 1977·Journal of Clinical Microbiology·P A MickelsenM E Mangum
Jun 29, 1978·The New England Journal of Medicine·T A PheiferK K Holmes
Feb 1, 1979·Journal of Clinical Microbiology·J R Greenwood, M J Pickett
Jan 1, 1979·Journal of Clinical Microbiology·R K BaileyR F Smith
May 1, 1979·The Journal of Clinical Investigation·K C ChenK K Holmes
Sep 1, 1975·Journal of Clinical Microbiology·B H MaloneL V Holdeman
Oct 1, 1969·Journal of Bacteriology·C W Moss, W E Dunkelberg
Sep 11, 1980·The New England Journal of Medicine·C A SpiegelK K Holmes
Jun 1, 1980·American Journal of Obstetrics and Gynecology·H L Gardner
Nov 19, 1954·Science·H L GARDNER, C D DUKES

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