Method for macromolecular colocalization using atomic recombination in dynamic SIMS

The Journal of Physical Chemistry. B
Guillaume LegentCamille Ripoll

Abstract

Localizing two or more components of assemblies in biological systems requires both continued development of fluorescence techniques and invention of entirely new techniques. Candidates for the latter include dynamic secondary ion mass spectrometry (D-SIMS). The latest generation of D-SIMS, the Cameca NanoSIMS 50, permits the localization of specific, isotopically labeled molecules and macromolecules in sections of biological material with a resolution in the tens of nanometers and with a sensitivity approaching in principle that of a single protein. Here we use two different systems, crystals of glycine and mixtures of proteins, to show that the formation of recombinant CN secondary ions under Cs bombardment can be exploited to create a new colocalization technique. We show experimentally that the formation of the recombinant (13)C(15)N secondary ion between (13)C- and (15)N-labeled macromolecules is indeed an indicator of the distance between the interacting macromolecules and on their shape. We build up a convolution model of the mixing-recombination process in D-SIMS that allows quantitative interpretations of the distance-dependent formation of the recombinant CN. Our results show that macromolecules can be colocalized if ...Continue Reading

References

Jan 1, 1992·Biology of the Cell·G SlodzianF Hillion
May 5, 1986·Journal of Molecular Biology·F W Studier, B A Moffatt
Dec 11, 1999·Nature·L H HartwellA W Murray
Mar 1, 2000·Analytical Chemistry·S ChandraG H Morrison
Jun 6, 2006·Journal of the American Society for Mass Spectrometry·G McMahonC J Unkefer

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Citations

Mar 9, 2011·Theory in Biosciences = Theorie in Den Biowissenschaften·Vic NorrisAlain R Thierry
Aug 20, 2011·Analytical Chemistry·Armelle Cabin-FlamanVic Norris
Dec 18, 2008·Annual Review of Biophysics·Steven G BoxerPeter K Weber
May 24, 2012·Chembiochem : a European Journal of Chemical Biology·Kensuke GotoMitsutoshi Setou
Oct 20, 2018·The Analyst·Lei YinJingkai Gu
Apr 28, 2017·Journal of Molecular Microbiology and Biotechnology·Vic NorrisGlenn J White
Oct 24, 2020·Theory in Biosciences = Theorie in Den Biowissenschaften·Yoan Konto-Ghiorghi, Vic Norris

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