Method for Postmortem Tissue Quantification of Δ9-Tetrahydrocannabinol and Metabolites Using LC-MS-MS

Journal of Analytical Toxicology
Ahmed I Al-Asmari

Abstract

A method for analyzing Δ9-tetrahydrocannabinol (THC), 11-hydroxy-Δ9-THC (THC-OH), and 11-nor-Δ9-THC-9-carboxylic acid (THC-COOH) in postmortem solid specimens using liquid chromatography-tandem mass spectrometry (LC-MS-MS) was developed and validated. A Stomacher instrument was used to prepare these tissues before extraction. Prior to solid phase extraction, liver, kidney, stomach, lung, brain, muscle, bladder and intestine tissues were pretreated with alkaline hydrolysis. All calibration curves were found to be linear with coefficients of determination greater than 0.999. The limit of quantification was 1.0 ng/g. Using three controls, within-run precision ranged between 1.0 and 12.0%, between-run precision ranged between 1.0 and 6.0%, and accuracy ranged between -7.0 and 8.0%. Matrix effects ranged from -21 to 24%. After matrix effects were excluded, analytical recoveries ranged from 79 to 97%. The distributions of THC, THC-OH, and THC-COOH were investigated in 32 postmortem cases that tested positive for cannabinoids. This revealed new information regarding the distribution of THC metabolites in stomach, intestine, and bladder. Alkaline hydrolysis was sufficient for the deglucuronidation of THC-COOH-glucuronide to its free fo...Continue Reading

References

Mar 1, 1995·Journal of Analytical Toxicology·K KudoN Jitsufuchi
Dec 17, 2005·Journal of Analytical Toxicology·Patrick MuraThierry Hauet
May 17, 2011·Forensic Science International : Synergy·Antonia Gronewold, Gisela Skopp
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Aug 24, 2017·Journal of Analytical Toxicology·Sunday R SaenzJarrad R Wagner
Oct 31, 2019·Journal of Analytical Toxicology·Ahmed I Al-Asmari

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