PMID: 3727490Jan 1, 1986

Method for processing leukocyte- and platelet-poor red cells in closed bags

Vox Sanguinis
T ShimizuS Goto

Abstract

If leukocyte- and platelet-poor red cells have been processed and stored in closed bags, their delivery, especially on holidays, will be easy. The modified warm-centrifuge method was applied to a quadruple bag prepared for such purpose. Red cells were processed in the first bag by the centrifugation of whole blood. The supernatant plasma was transferred to the second bag. A phosphate buffer in the third bag was introduced into red cells and diluted red cells were then incubated at 37 degrees C for 1 h in an inverted position. After centrifugation, the red cell phase below the buffy coat layer was isolated using a Biotest separation apparatus and stored in the fourth bag (containing a preservative with adenine, phosphate, glucose and 0.9% NaCl solution) for 28 days at 4 degrees C. Ninety-eight percent of the leukocyte and 97% of platelets in whole blood unit were removed with a red cell recovery of 86%. Biochemical changes in stored red cells were similar to those of conventional buffy coat-removed red cells suspended in the same preservative, except for rapid decreases in 2,3-diphosphoglycerate levels. Since processing buffy coat-removed red cells containing few leukocytes and platelets with the present method was simple and th...Continue Reading

References

Jul 1, 1974·Transfusion·F DeVenutoS M Wilson
May 1, 1980·Transfusion·H T MerymanR Lebovitz
Jan 1, 1964·Acta Physiologica Scandinavica·C H DEVERDIERJ KILLANDER

Related Concepts

Adenosine Triphosphate, Chromium Ammonium Salt
Apheresis
Blood Glucose
Blood Preservation
Hemolysis
Lymphapheresis
Therapeutic Plateletpheresis
Potassium
Sodium

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