Method for screening compounds that influence virulence gene expression in Staphylococcus aureus.

Antimicrobial Agents and Chemotherapy
Anita NielsenHanne Ingmer

Abstract

We present a simple assay to examine effects of compounds on virulence gene expression in the human pathogen Staphylococcus aureus. The assay employs transcriptional reporter strains carrying lacZ fused to central virulence genes. Compounds affecting virulence gene expression and activity of the agr locus are scored based on color change in the presence of a chromogenic beta-galactosidase substrate. The assay can be used to screen for novel antivirulence compounds from many different sources, such as fungi, as demonstrated here.

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Citations

Mar 9, 2013·Microbiology and Molecular Biology Reviews : MMBR·Breah LaSarre, Michael J Federle
Nov 26, 2013·Future Microbiology·Nadja B Cech, Alexander R Horswill
Nov 21, 2013·The Lancet Infectious Diseases·Ramanan LaxminarayanOtto Cars
Mar 14, 2012·FEMS Immunology and Medical Microbiology·Deirdre Fitzgerald-HughesHilary Humphreys
Jan 8, 2015·Frontiers in Microbiology·Cassandra L Quave, Alexander R Horswill
Feb 25, 2018·Scientific Reports·Georgia KarathanasiHanne Ingmer
Aug 31, 2017·Scientific Reports·Tim H JakobsenMichael Givskov
Apr 9, 2020·Frontiers in Microbiology·Clovis Macêdo Bezerra FilhoMaria Luiza Vilela Oliva

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