Method for the analysis in maize of the Fusarium mycotoxin moniliformin employing ion-pairing extraction and high-performance liquid chromatography

Journal of Chromatography
M J Shepherd, J Gilbert

Abstract

The Fusarium mycotoxin moniliformin (hydroxycyclobutenedione) has been determined in maize using a novel method with a recovery of 70-80% at 400-1600 micrograms/kg and 60% at the detection limit of 100 micrograms/kg. The method requires extraction of the toxin into aqueous tetra-n-butylammonium hydroxide and removal of cations from this solution by ion-exchange chromatography. Following clean-up by partitioning against dichloromethane, further quaternary ammonium reagent was added to the aqueous phase which was absorbed onto a hydrophilic matrix and the tetra-n-butylammonium moniliformate ion pair extracted into dichloromethane. After evaporation of the organic eluent, the residue was dissolved in aqueous sodium chloride and moniliformin quantitated by ion-pair high-performance liquid chromatography with UV detection. A batch of five samples may be analysed in 5-6 h including the chromatographic determination.

Citations

Sep 1, 1993·Mycotoxin Research·H LewW Edinger
Mar 21, 2012·Journal of Agricultural and Food Chemistry·Katharina Walburga von BargenHans-Ulrich Humpf
Sep 17, 2004·Food Additives and Contaminants·C M Maragos
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