Method for the extraction of the volatile compound salicylic acid from tobacco leaf material

Phytochemical Analysis : PCA
Marianne C VerberneRobert Verpoorte

Abstract

Salicylic acid (SA) is a signalling compound in plants which is able to induce systemic acquired resistance. In the analysis of SA in plant tissues, the extraction recovery is often very low and variable. This is mainly caused by sublimation of SA, especially during evaporation of organic solvents. Techniques have been designed in order to overcome this problem. In the first part of the extraction procedure, sublimation of SA was prevented by addition of 0.2 M sodium hydroxide. At a later stage of the extraction procedure, sublimation of SA during solvent evaporation was controlled by the addition of a small amount of HPLC eluent. In this way, recoveries in the range of 71-91% for free SA and 65-79% for acid-hydrolysed SA were obtained. Recoveries could be further optimised by the use of an internal standard to correct for volume changes after the addition of the HPLC eluent.

References

Mar 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·A J EnyediI Raskin
Aug 3, 1999·Nature Biotechnology·L ChaerleD Van Der Straeten
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·I RaskinW R Melander

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Citations

Mar 23, 2011·Journal of Plant Research·Sota KoedaSusumu Yazawa
Jun 12, 2013·Molecular Plant-microbe Interactions : MPMI·Vijayata SinghAshis Kumar Nandi
Sep 25, 2010·Plant Methods·George MarekZhonglin Mou
Mar 29, 2014·Planta·Danuše TarkowskáMiroslav Strnad
Feb 10, 2009·Plant Molecular Biology·Francisca BlancoLoreto Holuigue
Aug 14, 2010·Journal of Plant Physiology·Sanimah SimohRobert Verpoorte
Mar 31, 2009·Plant Physiology and Biochemistry : PPB·Sudhamoy MandalAdinpunya Mitra
Jul 2, 2003·The Plant Journal : for Cell and Molecular Biology·Marianne C VerberneHuub J M Linthorst
Sep 20, 2008·The Plant Journal : for Cell and Molecular Biology·Vincentius A HalimSabine Rosahl
Nov 28, 2008·The Plant Journal : for Cell and Molecular Biology·Zuyu ZhengZhixiang Chen
Feb 25, 2009·Molecular Plant Pathology·Nicolás Miguel CecchiniMaría Elena Alvarez
Apr 21, 2016·Plant Science : an International Journal of Experimental Plant Biology·Géraldine van AubelPierre Van Cutsem
Sep 3, 2010·The Plant Journal : for Cell and Molecular Biology·Christopher T DeFraiaZhonglin Mou
Oct 13, 2011·The New Phytologist·Consolación ÁlvarezIrene García
May 4, 2011·The Plant Journal : for Cell and Molecular Biology·In Sun HwangByung Kook Hwang
May 4, 2011·Journal of Integrative Plant Biology·Chuanfu An, Zhonglin Mou
Feb 13, 2016·The Plant Journal : for Cell and Molecular Biology·Yezhang DingZhonglin Mou
Mar 5, 2016·The Plant Journal : for Cell and Molecular Biology·Ranabir SahuShree P Pandey
Mar 31, 2015·Transgenic Research·Katchen Julliany P SilvaKevin M Folta
Jan 23, 2015·Frontiers in Plant Science·Yezhang DingZhonglin Mou

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