PMID: 9160421Jan 1, 1997Paper

Method for the growth of equine airway epithelial cells in culture

Research in Veterinary Science
A SimeA Nolan

Abstract

A serum-free cell culture method was developed for equine tracheal epithelial cells which allowed the growth and characterisation of the phenotypical properties of this cell type. Several variables influenced the efficacy of the attachment and growth of the isolated cells. Serum and a collagen matrix were essential components for efficient cell attachment. Once attachment had occurred, cell growth was enhanced by a serum-free medium containing bovine pituitary extract, retinoic acid, insulin, hydrocortisone, transferrin, epidermal growth factor, adrenaline and triiodothyronine. The mean time taken for the cells to grow to confluency varied from 12.6 to 28.0 days, depending on the medium used. Collagen matrix was essential to aid the proliferation of the cells.

References

Jan 1, 1992·American Journal of Respiratory Cell and Molecular Biology·P R GrayM L Peters-Golden
Jan 1, 1986·Experimental Lung Research·M R Van ScottR C Boucher
Jan 1, 1984·Experimental Lung Research·T C LeeP Nettesheim

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Citations

Jul 3, 2008·In Vitro Cellular & Developmental Biology. Animal·Workineh ShibeshiFritz R Ungemach
Nov 17, 2009·In Vitro Cellular & Developmental Biology. Animal·Ute E SchwabDavid G Russell
Apr 25, 2008·Expert Opinion on Drug Metabolism & Toxicology·Jennifer L SportyCarsten Ehrhardt
Jan 9, 2013·Expert Opinion on Drug Metabolism & Toxicology·Xin LiuMichael S Roberts
Oct 15, 2005·In Vitro Cellular & Developmental Biology. Animal·Susan M LankfordLinda D Martin
Dec 19, 2018·Wiley Interdisciplinary Reviews. Developmental Biology·Arthur LuhurAndrew C Zelhof
Mar 21, 2002·Equine Veterinary Journal·A M DahmM M Suter

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