Method for the identification and quantification of sixty drugs and their metabolites in postmortem whole blood using liquid chromatography tandem mass spectrometry

Forensic Science International : Synergy
Ahmed I Al-Asmari

Abstract

The aim of this work was to develop and validate a liquid chromatography tandem mass spectrometry method for detecting sixty drugs and metabolites that are most commonly encountered in postmortem whole blood analysis. Although a large number of drugs were included in the panel, acceptance criteria for method validation were achieved. All calibration curves were found to be linear with coefficients of determination greater than 0.99. The limits of detection ranged from 0.2ng/mL to 1.0ng/mL and the limits of quantification range from 1.0ng/mL to 5.0ng/mL. Using three controls, within-run precision was 0.7%-10.3% and between-run precision was 0.6%-9.0%. Accuracy was ranged from 95.0%-104.1%. Matrix effects ranged from -15% to +22%. After excluding matrix effects, analytical recoveries ranged from 76% to 100%. Coefficients of variation for matrix effects ranged from 0.5%-13% and coefficients of variation for recovery ranged from 0.9%-13.0%. Over 1000 postmortem blood samples were analyzed. Among them, 435 cases (45%) tested positive for at least one analyte of interest. In conclusion, this study presents a technique for multianalyte screening of sixty drugs and metabolites that are commonly encountered in postmortem toxicology. Thi...Continue Reading

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Citations

Jan 2, 2021·Forensic Science International : Synergy·Jacqueline de M CampêloJandyson M Santos
Mar 7, 2021·Forensic Science International : Synergy·Ahmed I Al-Asmari

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