PMID: 5904976Jan 1, 1966

Method of assay of red cell folate activity and the value of the assay as a test for folate deficiency

Journal of Clinical Pathology
A V HoffbrandD L Mollin


A simplified microbiological assay for determining the folate content of red cells is described. As in previously reported methods Lactobacillus casei is used as test organism but two modifications are introduced. First, haemolysis is carried out in water containing 1 g.% of ascorbic acid; secondly, haemolysates are not incubated before the assay. Using this assay, recovery of pteroylglutamic acid added in two different concentrations to five different whole blood samples was 97.0 +/- 1.9 S.E. % and 106.1 +/- 4.7 S.E. % respectively. The coefficient of variation of the assay was between 11.2 and 15.0%. Haemolysates were best stored deep frozen, showing no significant loss of L. casei activity for three to five months at -20 degrees C. On the other hand, non-haemolysed blood samples were best stored at 4 degrees C. when there was no loss of activity for seven to 10 days. Experiments confirmed that plasma is necessary for the maximum release of red cell L. casei activity, and showed that only small amounts of plasma are necessary; folate- and B(12)-deficient plasma released slightly lower L. casei activities from red cells than did normal plasma. The red cell folate levels of 40 healthy normal subjects ranged from 160 to 640 mmug...Continue Reading


Feb 1, 1959·Proceedings of the Society for Experimental Biology and Medicine·H P BROQUIST, A L LUHBY
May 1, 1959·Journal of Clinical Pathology·M M BODDINGTON, A I SPRIGGS
Jun 1, 1959·Proceedings of the Society for Experimental Biology and Medicine·A L LUHBYD N TELLER
Jul 1, 1961·Archives of Internal Medicine·F C CARTERR J KORN
Jul 1, 1960·British Journal of Haematology·N GrossowiczB BERCOVICI
Jan 1, 1961·The Journal of Clinical Investigation·V Herbert
Jul 1, 1961·Journal of Clinical Pathology·A H Waters, D L Mollin
Jan 6, 1962·British Medical Journal·I Chanarin, M C BENNETT
Jun 1, 1962·The Journal of Clinical Investigation·V Herbert, R ZALUSKY
Jun 1, 1963·Archives of Biochemistry and Biophysics·J M NORONHA, V S ABOOBAKER
Nov 1, 1962·Archives of Internal Medicine·V Herbert
Mar 1, 1963·Dapim refuiim. Folia medica·S BAR-SHANII MATOT
Jan 1, 1964·Journal of Clinical Pathology·B B Anderson
Jan 1, 1965·Annual Review of Medicine·V Herbert


Feb 1, 1972·Indian Journal of Pediatrics·M Gupta, K N Agarwal
Feb 1, 1985·Irish Journal of Medical Science·C P WickhamJ P Kevany
Jul 15, 1983·Clinica Chimica Acta; International Journal of Clinical Chemistry·N J FullerK J Scott
Jan 1, 1988·The International Journal of Biochemistry·R E Davis, D J Nicol
Jan 9, 1992·European Journal of Obstetrics, Gynecology, and Reproductive Biology·B LarroqueG Crépin
Jan 1, 1986·Transactions of the Royal Society of Tropical Medicine and Hygiene·S J Oppenheimer, P Cashin
Jan 1, 1991·Comparative Biochemistry and Physiology. A, Comparative Physiology·A DhurSerge Hercberg
Aug 27, 1970·The New England Journal of Medicine·J D Hines, D H Cowan
Jun 17, 1982·The New England Journal of Medicine·G F Longstreth, R Green
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·R E BeneschC M Baugh
May 1, 1972·The British Journal of Nutrition·J E FordK J Scott
Nov 1, 1974·The British Journal of Nutrition·R C Siddons
Mar 1, 1987·The British Journal of Nutrition·Serge HercbergG Potier de Courcy
Sep 1, 1968·The Proceedings of the Nutrition Society·J Andrews
Sep 1, 1965·British Journal of Haematology·S Kremenchuzky, A V Hoffbrand
Nov 1, 1967·British Journal of Haematology·A V Hoffbrand, B F Newcombe
Apr 1, 1968·British Journal of Haematology·C E Butterworth
Jan 1, 1969·British Journal of Haematology·I ChanarinV Wincour
Aug 1, 1970·British Journal of Haematology·K C Das, A V Hoffbrand
Feb 1, 1971·British Journal of Haematology·P D RobertsJ O Morgan
Sep 1, 1972·British Journal of Haematology·A V Hoffbrand, A H Waters
Jun 1, 1973·British Journal of Haematology·V HerbertL Brenner
Sep 1, 1973·British Journal of Haematology·I ChanarinA V Hoffbrand
Aug 1, 1974·British Journal of Haematology·C Schreiber, S Waxman
Sep 1, 1975·British Journal of Haematology·M P Westerman
Oct 1, 1969·Archives of Disease in Childhood·P M RobertsM E Monk-Jones
Apr 1, 1971·Archives of Disease in Childhood·W L BurlandJ Lord
Oct 1, 1971·Archives of Disease in Childhood·D M CookJ S Stewart
May 1, 1973·Archives of Disease in Childhood·M KhalilM el-Lozy
Sep 1, 1973·Archives of Disease in Childhood·E D Hibbard
Dec 1, 1974·Archives of Disease in Childhood·H B Valman, P D Roberts
Jan 29, 1972·British Medical Journal·J D MaxwellR Williams
Jul 23, 1966·British Medical Journal·P D RobertsD L Mollin
Apr 13, 1968·British Medical Journal·A V HoffbrandD L Mollin
Sep 6, 1969·British Medical Journal·F MurphyA Elwis
Aug 12, 1972·British Medical Journal·L FryA V Hoffbrand
Jul 14, 1973·British Medical Journal·P D RobertsA V Hoffbrand
Nov 3, 1973·British Medical Journal·J StuartD N Raine
Nov 1, 1976·Gut·G Thomas, D J Clain
Sep 1, 1967·Journal of Clinical Pathology·A V HoffbrandD L Mollin
Mar 1, 1969·Journal of Clinical Pathology·G H Spray
Mar 1, 1969·Journal of Clinical Pathology·A Omer
Feb 1, 1970·Journal of Clinical Pathology·R E DavisA Kelly
Feb 1, 1970·Journal of Clinical Pathology·L MillbankA H Waters
Nov 1, 1970·Journal of Clinical Pathology·F P Retief, Y J Huskisson
Apr 1, 1971·Journal of Clinical Pathology·J Forshaw, L Harwood
Aug 1, 1972·Journal of Clinical Pathology·P R Teasdale, J Pearce
Feb 1, 1976·Journal of Clinical Pathology·S C Tso
Jul 1, 1977·Journal of Clinical Pathology·I JohnsonM Rose
Jan 1, 1978·Journal of Clinical Pathology·I Johnson, M Rose
Oct 1, 1978·Journal of Clinical Pathology·L A HenshawM E Beard
Apr 1, 1992·Journal of Clinical Pathology·S D O'Broin, B P Kelleher
Dec 1, 1971·Journal of Neurology, Neurosurgery, and Psychiatry·E H ReynoldsA L Johnson
Jun 18, 1966·Lancet·A V HoffbrandD L Mollin
Jan 1, 1984·Critical Reviews in Clinical Laboratory Sciences·J Metz
Jun 1, 1974·The Journal of Obstetrics and Gynaecology of the British Commonwealth·R A MolinaN Velázquez
Dec 1, 1966·The Journal of Obstetrics and Gynaecology of the British Commonwealth·T M Vanier, J F Tyas
Apr 25, 1975·Annals of the New York Academy of Sciences·J D Hines
Sep 1, 1971·The Journal of Obstetrics and Gynaecology of the British Commonwealth·B M Hibbard, E D Hibbard
Nov 1, 1969·The Journal of Obstetrics and Gynaecology of the British Commonwealth·B M HibbardP Tan
Feb 1, 1974·The British Journal of Dermatology·L Fry, P P Seah
Jul 1, 1972·The Journal of Obstetrics and Gynaecology of the British Commonwealth·B M Hibbard, E D Hibbard
Nov 1, 1971·British Journal of Haematology·P C ElwoodA C Frazer
Oct 9, 2003·British Journal of Haematology·A V Hoffbrand
Dec 20, 2013·European Journal of Haematology·Edward De BruynFrédéric Cotton
May 1, 1973·The American Journal of Medicine·Y K Liu
May 1, 1992·The American Journal of Emergency Medicine·R A Schwab, R D Powers
Jul 1, 1983·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·J E SquiresA Shenkin
Sep 1, 1995·Baillière's Clinical Haematology·R Green
Feb 1, 1982·British Journal of Haematology·S E SteinbergR S Hillman
Oct 1, 1983·British Journal of Haematology·N L NelsonR F Branda
Jun 1, 1987·Baillière's Clinical Haematology·T E Parry
Jan 1, 1980·Clinical and Laboratory Haematology·T E Parry
May 1, 1974·The Journal of Pediatrics·E D HibbardA P Kenna
Sep 26, 2015·Seminars in Hematology·Kazusa Ishii, Neal S Young
Jul 1, 1982·Pathology·J G BuchananH Alexander
Jun 1, 1977·American Journal of Obstetrics and Gynecology·O Martinez, D A Roe
Dec 31, 2014·Analytical Chemistry·Riikka ArppeTero Soukka
Oct 1, 1979·Journal of the American Geriatrics Society·S G Webster, J T Leeming
Jun 26, 2001·Clinical and Laboratory Haematology·N PhilpottS D O'Broin
Jun 1, 1969·The Journal of Obstetrics and Gynaecology of the British Commonwealth·T Edelstein, J Metz
Oct 1, 1967·The American Journal of Medicine·J D HinesD L Mollin
Aug 1, 1993·Nutrition in Clinical Practice : Official Publication of the American Society for Parenteral and Enteral Nutrition·D K Bernard, M J Shaw
Feb 1, 1983·Journal of Cellular Physiology·S E SteinbergR S Hillman
Dec 1, 1971·Archives of Disease in Childhood·M J Landon, A Oxley
Jul 1, 1966·Journal of Clinical Pathology·G H Spray, I J Temperley
May 1, 1977·Journal of Clinical Pathology·P W Hill, D W Dawson
Mar 31, 2010·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Erika Coria-RamirezAbraham Majluf-Cruz
Aug 31, 2001·European Journal of Clinical Nutrition·M WartanowiczL Konopka
Aug 4, 2006·European Journal of Clinical Nutrition·N MitoN Yoshiike
Dec 1, 1985·Fortschritte der Kieferorthopädie·M M Rosé
Mar 1, 1975·The British Journal of Psychiatry : the Journal of Mental Science·R D EasthamJ D Cameron
Jul 2, 2019·The Cochrane Database of Systematic Reviews·Elizabeth Centeno TablanteJulia L Finkelstein
Jan 29, 2002·Coronary Artery Disease·L K MichalisD A Sideris
Jan 1, 1978·American Journal of Hematology·J FerrerB Rodríguez-Iturbe
Jun 29, 2000·Journal of the American College of Nutrition·J C Essama-TjaniD Richard
Mar 1, 1975·Scandinavian Journal of Haematology·Y K Liu
Feb 1, 1977·Scandinavian Journal of Haematology·T H WeberK Tontti
Jun 1, 1971·The British Journal of Dermatology·L FryA V Hoffbrand
Jan 1, 1967·Scandinavian Journal of Haematology·K LindstrandA V Hoffbrand
Feb 1, 1991·American Journal of Hematology·J P Jaffe, R F Schilling
Nov 1, 1994·The Proceedings of the Nutrition Society·J ScottD Weir
Mar 1, 1979·Scandinavian Journal of Haematology·S VatanavicharnP Wasi
Jan 1, 1981·Clinical and Laboratory Haematology·S N Wickramasinghe
Oct 1, 1973·The British Journal of Dermatology·R TouraineM Tulliez
May 23, 2001·Experimental Biology and Medicine·N M van der PutH J Blom
Apr 16, 1999·Journal of the American College of Nutrition·P PreziosiSerge Hercberg
Nov 1, 1972·Scottish Medical Journal·K P Dawson
Jan 1, 1971·Scandinavian Journal of Haematology·S D LawlerA V Hoffbrand
Sep 1, 1969·Scottish Medical Journal·R H Girdwood
Sep 9, 1999·The American Journal of Clinical Nutrition·S D O'Broin, E W Gunter
Sep 1, 1973·CRC Critical Reviews in Clinical Laboratory Sciences·H E SauberlichJ H Skala
Sep 30, 1998·European Journal of Haematology·A CafollaC S Scott
Aug 1, 1970·Scottish Medical Journal·I R Williams, R H Girdwood
Feb 1, 1979·Postgraduate Medicine·P C Ward
Jan 1, 1979·Acta geneticae medicae et gemellologiae·M H HallR J Davidson
Aug 1, 1989·American Journal of Hematology·H C FordM A Rendle
Jan 1, 1967·Scandinavian Journal of Haematology·T MarkkanenS Virtanen
Jan 1, 1980·International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics·S C Tso, R L Wong
Jul 20, 1968·The Medical Journal of Australia·A D Hurdle

Related Concepts

Anemia, Macrocytic
Anemia, Pernicious
Figlu Test
Folic Acid, Sodium Salt
Folic Acid Deficiency

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.