Method of salivary histatins determination.

Klinicheskaia laboratornaia diagnostika
V G ArzumanianA Yu Mironov

Abstract

Histatins are the most significant antimicrobial peptides (AMP) of saliva and there are 12 types of such AMP. Histatin molecules contain relatively high percent of histidine and tyrosine residues. This property allows to use well known from organic chemistry Pauly reaction for detection of protein bounded histidine and tyrosine residues (BHT), which are in fact characterize the summary content of all histatins in saliva. Aim of the present study was comparison of BHT with antimicrobial activity of salivary AMP fraction in patients with inflammatory diseases of upper airways (IDUA). Group of examined persons include 28 patients with different diagnoses: chronic pharyngitis (n=11), chronic tonsillitis (n=7), nasopharyngitis (n=5), pollinosis (n=5). Degree of intensity of inflammatory symptoms was estimated in balls. The algorithm of BHT analysis include following steps: freezing - thawing of saliva; removal of microparticles by centrifugation; separation of fraction lower than100 kDa; dialysis for free amino acids removal; Pauly reaction carrying out. Antimicrobial activities of saliva and its low molecular fractions were estimated towards Candida albicans cells by the spectrophotometric method with bromocresol purpur. Analysis o...Continue Reading

References

Dec 1, 1986·Journal of Clinical Microbiology·J W JenzanoR L Lundblad
Feb 1, 1995·European Journal of Oral Sciences·M CuidaR Jonsson
Nov 24, 1998·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·H Tsai, L A Bobek
Jan 23, 2009·Archives of Oral Biology·Melanie CampeseEva J Helmerhorst
Apr 3, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xiuli SunEva J Helmerhorst
Oct 19, 2016·Saudi Pharmaceutical Journal : SPJ : the Official Publication of the Saudi Pharmaceutical Society·Zohaib KhurshidMuhammad Sohail Zafar
Feb 23, 2017·Saudi Pharmaceutical Journal : SPJ : the Official Publication of the Saudi Pharmaceutical Society·Zohaib KhurshidMuhammad Sohail Zafar
Jun 3, 2017·International Journal of Dentistry·Deepak Gowda Sadashivappa PateelSreejith Muraleedharan Nair

❮ Previous
Next ❯

Related Concepts

Related Feeds

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.